ArticleThe American journal of pathology2002
Up-regulation of connexin43 in glomerular podocytes in response to injury.
Article in The American journal of pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Genetic and nongenetic factors affecting vancomycin population pharmacokinetics and pharmacodynamics: A systematic review.Indian journal of pharmacology · 2026Pooled it
- Podocyte injury damages podocytes in chimeric organoids.PloS one · 2026Article
- The Role of Connexin 43 in Renal Disease: Insights from In Vivo Models of Experimental Nephropathy.International journal of molecular sciences · 2022Review
- Connexin mRNA distribution in adult mouse kidneys.Pflugers Archiv : European journal of physiology · 2021Article
- Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing.International journal of molecular sciences · 2021Review
- Podocytes and microRNA-30/Cx43 axis in diabetic nephropathy.Annals of translational medicine · 2021Article
- Intercellular transmission of endoplasmic reticulum stress through gap junction targeted by microRNAs as a key step of diabetic kidney diseases?Annals of translational medicine · 2021Article
- MicroRNA-30/Cx43 axis contributes to podocyte injury by regulating ER stress in diabetic nephropathy.Annals of translational medicine · 2020Article
- Novel Targets for Therapy of Renal Fibrosis.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Review
- Hydrogen Sulfide Protects Hyperhomocysteinemia-Induced Renal Damage by Modulation of Caveolin and eNOS Interaction.Scientific reports · 2019Article
- Morphological Processes of Foot Process Effacement in Puromycin Aminonucleoside Nephrosis Revealed by FIB/SEM Tomography.Journal of the American Society of Nephrology : JASN · 2019Article
- Risedronate Attenuates Podocyte Injury in Phosphate Transporter-Overexpressing Rats.International journal of endocrinology · 2019Article
- Decreased Expression of Connexin 43 Blunts the Progression of Experimental GN.Journal of the American Society of Nephrology : JASN · 2017Article
- Mind the gap: connexins and cell-cell communication in the diabetic kidney.Diabetologia · 2015Review
- Article
- Intravital imaging of podocyte calcium in glomerular injury and disease.The Journal of clinical investigation · 2014Article
- The importance of podocyte adhesion for a healthy glomerulus.Frontiers in endocrinology · 2014Review
- Defining cell-type specificity at the transcriptional level in human disease.Genome research · 2013Article
- The podocyte slit diaphragm--from a thin grey line to a complex signalling hub.Nature reviews. Nephrology · 2013Review
- Molecular understanding of the slit diaphragm.Pediatric nephrology (Berlin, Germany) · 2013Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podocyte injury or podocyte loss in the renal glomerulus has been proposed as the crucial mechanism in the development of focal segmental glomerulosclerosis. However, it is poorly understood how podocytes respond to injury. In this study, glomerular expression of connexin43 (Cx43) gap junction protein was examined at both protein and transcript levels in an experimental model of podocyte injury, puromycin aminonucleoside (PAN) nephrosis. A striking increase in the number of immunoreactive dots with anti-Cx43 antibody was demonstrated along the glomerular capillary wall in the early to nephrotic stage of PAN nephrosis. The conspicuous change was not detected in the other areas including the mesangium and Bowman's capsule. Immunoelectron microscopy showed that the immunogold particles for Cx43 along the capillary wall were localized predominantly at the cell-cell contact sites of podocytes. Consistently, Western blotting and ribonuclease protection assay revealed a distinct increase of Cx43 protein, phosphorylation, and transcript in glomeruli during PAN nephrosis. The changes were detected by 6 hours after PAN injection. These findings indicate that the increase of Cx43 expression is one of the earliest responses that have ever been reported in podocyte injury. To show the presence of functional gap junctional intercellular communication (GJIC) in podocytes, GJIC was assessed in podocytes in the primary culture by transfer of fluorescent dye, Lucifer yellow, after a single-cell microinjection. Diffusion of the dye into adjacent cells was observed frequently in the cultured podocytes, but scarcely in cultured parietal epithelial cells of Bowman's capsule, which was compatible with their Cx43 staining. Thus, it is concluded that Cx43-mediated GJIC is present between podocytes, suggesting that podocytes may respond to injury as an integrated epithelium on a glomerulus rather than individually as a separate cell.
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