ArticleThe EMBO journal2002
Ribonuclease activity and RNA binding of recombinant human Dicer.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 213 papers.
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Who cites it
213 citing papers in PubMed, 451 citations in OpenAlex.
- How Do DICER1 Syndrome Mutations Disrupt Catalysis? Unveiling Dicer Metal Binding Architecture and Mechanism of Action Using MD Simulations and QM/MM Calculations.Journal of computational chemistry · 2026Article
- Response to "Antiviral RNA silencing in mammals: the importance of selecting the appropriate experimental model".Nucleic acids research · 2025Article
- Clinical and genetic features of a family with multinodular goiter harboring a DICER1 c.2035 A >G variant.Thyroid research · 2025Article
- RNA Through Time: From the Origin of Life to Therapeutic Frontiers in Transcriptomics and Epitranscriptional Medicine.International journal of molecular sciences · 2025Review
- The biogenesis and regulation of animal microRNAs.Nature reviews. Molecular cell biology · 2025Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- DICER1 platform domain missense variants inhibit miRNA biogenesis and lead to tumor susceptibility.NAR cancer · 2023Article
- Article
- The Integrator complex regulates microRNA abundance through RISC loading.Science advances · 2023Article
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.Molecular cell · 2022Article
- Molecular map of chronic lymphocytic leukemia and its impact on outcome.Nature genetics · 2022Article
- Article
- stepRNA: Identification of Dicer cleavage signatures and passenger strand lengths in small RNA sequences.Frontiers in bioinformatics · 2022Article
- Untangling the roles of RNA helicases in antiviral innate immunity.PLoS pathogens · 2021Review
- The Challenges and Opportunities in the Development of MicroRNA Therapeutics: A Multidisciplinary Viewpoint.Cells · 2021Review
- Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2.Nature communications · 2021Article
- Modulation of microRNA processing by 5-lipoxygenase.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Phase separation of SERRATE drives dicing body assembly and promotes miRNA processing in Arabidopsis.Nature cell biology · 2021Article
153 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing phenomena, known as post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference (RNAi) in animals, are mediated by double-stranded RNA (dsRNA) and mechanistically intersect at the ribonuclease Dicer. Here, we report cloning and expression of the 218 kDa human Dicer, and characterization of its ribonuclease activity and dsRNA-binding properties. The recombinant enzyme generated approximately 21-23 nucleotide products from dsRNA. Processing of the microRNA let-7 precursor by Dicer produced an apparently mature let-7 RNA. Mg(2+) was required for dsRNase activity, but not for dsRNA binding, thereby uncoupling these reaction steps. ATP was dispensable for dsRNase activity in vitro. The Dicer.dsRNA complex formed at high KCl concentrations was catalytically inactive, suggesting that ionic interactions are involved in dsRNA cleavage. The putative dsRNA-binding domain located at the C-terminus of Dicer was demonstrated to bind dsRNA in vitro. Human Dicer expressed in mammalian cells colocalized with calreticulin, a resident protein of the endoplasmic reticulum. Availability of the recombinant Dicer protein will help improve our understanding of RNA silencing and other Dicer-related processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.