ArticleThe EMBO journal2002
Structure of the sporulation-specific transcription factor Ndt80 bound to DNA.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
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- Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinations.bioRxiv : the preprint server for biology · 2025Article
- Transcription factor VIB-1 activates catalase-3 expression by promoting PIC assembly in Neurospora crassa.Nucleic acids research · 2025Article
- Gametogenesis: Exploring an Endogenous Rejuvenation Program to Understand Cellular Aging and Quality Control.Annual review of genetics · 2022Review
- Cadmium Sulfide Quantum Dots Adversely Affect Gametogenesis inNanomaterials (Basel, Switzerland) · 2022Article
- Structural basis of DNA methylation-dependent site selectivity of the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1.Nucleic acids research · 2022Article
- The meiotic-specific Mek1 kinase in budding yeast regulates interhomolog recombination and coordinates meiotic progression with double-strand break repair.Current genetics · 2019Review
- Mek1 coordinates meiotic progression with DNA break repair by directly phosphorylating and inhibiting the yeast pachytene exit regulator Ndt80.PLoS genetics · 2018Article
- The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.Nature communications · 2018Article
- Selective base excision repair of DNA damage by the non-base-flipping DNA glycosylase AlkC.The EMBO journal · 2018Article
- DNA-binding domain of myelin-gene regulatory factor: purification, crystallization and X-ray analysis.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Crystal structure of the DNA-binding domain of Myelin-gene Regulatory Factor.Scientific reports · 2017Article
- Coordination of Double Strand Break Repair and Meiotic Progression in Yeast by a Mek1-Ndt80 Negative Feedback Loop.Genetics · 2017Article
- Myrf ER-Bound Transcription Factors Drive C. elegans Synaptic Plasticity via Cleavage-Dependent Nuclear Translocation.Developmental cell · 2017Article
- Ndt80 activates the meiotic ORC1 transcript isoform and SMA2 via a bi-directional middle sporulation element in Saccharomyces cerevisiae.RNA biology · 2016Article
- Molecular insights into DNA binding and anchoring by the Bacillus subtilis sporulation kinetochore-like RacA protein.Nucleic acids research · 2016Article
- Differential Deformability of the DNA Minor Groove and Altered BI/BII Backbone Conformational Equilibrium by the Monovalent Ions Li(+), Na(+), K(+), and Rb(+) via Water-Mediated Hydrogen Bonding.Journal of chemical theory and computation · 2015Article
- VibA, a homologue of a transcription factor for fungal heterokaryon incompatibility, is involved in antifungal compound production in the plant-symbiotic fungus Epichloë festucae.Eukaryotic cell · 2015Article
- Identification and functional characterization of Rca1, a transcription factor involved in both antifungal susceptibility and host response in Candida albicans.Eukaryotic cell · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Progression through the middle phase of sporulation in Saccharomyces cerevisiae is promoted by the successful completion of recombination at the end of prophase I. Completion of meiotic recombination allows the activation of the sporulation-specific transcription factor Ndt80, which binds to a specific DNA sequence, the middle sporulation element (MSE), and activates approximately 150 genes to enable progression through meiosis. Here, we isolate the DNA-binding domain of Ndt80 and determine its crystal structure both free and in complex with an MSE-containing DNA. The structure reveals that Ndt80 is a member of the Ig-fold family of transcription factors. The structure of the DNA-bound form, refined at 1.4 A, reveals an unexpected mode of recognition of 5'-pyrimidine- guanine-3' dinucleotide steps by arginine residues that simultaneously recognize the 3'-guanine base through hydrogen bond interactions and the 5'-pyrimidine through stacking/van der Waals interactions. Analysis of the DNA-binding affinities of MSE mutants demonstrates the central importance of these interactions, and of the AT-rich portion of the MSE. Functional similarities between Ndt80 and the Caenorhabditis elegans p53 homolog suggest an evolutionary link between Ndt80 and the p53 family.
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