ArticleProceedings of the National Academy of Sciences of the United States of America2002
Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.Chemical science · 2021Article
- Development and optimization of heavy metal lead biosensors in biomedical and environmental applications.Journal of the Chinese Medical Association : JCMA · 2021Review
- Ab Initio Derivation of the FRET Equations Resolves Old Puzzles and Suggests Measurement Strategies.Biophysical journal · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in 11 different cell lines.Royal Society open science · 2017Article
- Farnesylthiosalicylic acid sensitizes hepatocarcinoma cells to artemisinin derivatives.PloS one · 2017Article
- Small Molecule Active Site Directed Tools for Studying Human Caspases.Chemical reviews · 2015Review
- Fluorescent proteins as genetically encoded FRET biosensors in life sciences.Sensors (Basel, Switzerland) · 2015Review
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics.Nature reviews. Cancer · 2014Review
- Application of FRET probes in the analysis of neuronal plasticity.Frontiers in neural circuits · 2013Review
- Resveratrol induces mitochondria-mediated AIF and to a lesser extent caspase-9-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells.Molecular and cellular biochemistry · 2011Article
- Cell death goes LIVE: technological advances in real-time tracking of cell death.Cell cycle (Georgetown, Tex.) · 2010Review
- Imaging and photodynamic therapy: mechanisms, monitoring, and optimization.Chemical reviews · 2010Review
- Plant caspase-like proteases in plant programmed cell death.Plant signaling & behavior · 2009Article
- Article
- Measuring apoptosis at the single cell level.Methods (San Diego, Calif.) · 2008Review
- Optical molecular imaging for systems biology: from molecule to organism.Analytical and bioanalytical chemistry · 2006Review
- An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.The EMBO journal · 2004Article
- Fluorescent probes for proteolysis: tools for drug discovery.Nature reviews. Drug discovery · 2004Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When cells are exposed to death-inducing molecules such as tumor necrosis factor-alpha or Fas, caspase 8 is activated and cleaves an apoptotic facilitator, Bid, that is a member of the Bcl-2 family. After additional modification, the C-terminal moiety of Bid is translocated to the mitochondria and induces the release of cytochrome c into the cytoplasm. In an attempt to directly observe the cleavage of Bid and the following events in living cells, we constructed a vector that encoded Bid fused with yellow fluorescent protein (YFP) and cyan fluorescent protein (CFP) (YFP-Bid-CFP). On expression of YFP-Bid-CFP in mammalian cells, we were able to observe the efficient transfer of energy from excited CFP to YFP within the YFP-Bid-CFP molecule and, importantly, the fusion protein YFP-Bid-CFP was fully functional in cells. When YFP-Bid-CFP was cleaved by caspase 8, on activation by anti-Fas Abs but not by Abeta or tunicamycin, no such transfer of energy was detected. To our knowledge, this is the first report of (i) visualization of the activation of Bid by proteolytic cleavage, with direct observation of the cleavage of YFP-Bid-CFP in the cytoplasm and subsequent translocation of the cleaved Bid to mitochondria and (ii) the absence of Abeta- or tunicamycin-mediated significant activation of caspase 8 in individual living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.