ArticleNucleic acids research2002
Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 170 papers, 1 of them a synthesis that pooled it.
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Who cites it
170 citing papers in PubMed, 1 synthesis or guideline pooled it, 646 citations in OpenAlex.
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- Harnessing Contact-Quenched, Profluorescent Chemical Probes for Sensitive Determination and High-Throughput Measurements of Enzyme Activity.ACS omega · 2025Article
- Fluorescent modifications in aptamer switches-positional, structural, and neighboring pair effects on sensor performance.Nucleic acids research · 2025Article
- Cleavable Hydrophobic Anchors Optimize Immunomodulatory Effects of Oligonucleotides.Journal of the American Chemical Society · 2025Article
- Tunable fluorogenic DNA probes drive fast and high-resolution single-molecule fluorescence imaging.Nucleic acids research · 2025Article
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- Subunit Communication within Dimeric SF1 DNA Helicases.Journal of molecular biology · 2024Article
- A non-FRET DNA reporter that changes fluorescence colour upon nuclease digestion.Nature nanotechnology · 2024Article
- Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing.Nature communications · 2024Article
- Heterochiral modifications enhance robustness and function of DNA in living human cells.Chembiochem : a European journal of chemical biology · 2024Article
- A Bioorthogonal Antidote Against the Photosensitivity after Photodynamic Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Thermal-plex: fluidic-free, rapid sequential multiplexed imaging with DNA-encoded thermal channels.Nature methods · 2024Article
110 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An important consideration in the design of oligonucleotide probes for homogeneous hybridization assays is the efficiency of energy transfer between the fluorophore and quencher used to label the probes. We have determined the efficiency of energy transfer for a large number of combinations of commonly used fluorophores and quenchers. We have also measured the quenching effect of nucleotides on the fluorescence of each fluorophore. Quenching efficiencies were measured for both the resonance energy transfer and the static modes of quenching. We found that, in addition to their photochemical characteristics, the tendency of the fluorophore and the quencher to bind to each other has a strong influence on quenching efficiency. The availability of these measurements should facilitate the design of oligonucleotide probes that contain interactive fluorophores and quenchers, including competitive hybridization probes, adjacent probes, TaqMan probes and molecular beacons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.