ArticleNucleic acids research2002
The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Fission yeast Rad54 prevents intergenerational buildup of Rad51 aggregates in proliferating cells.Life science alliance · 2025Article
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- Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.Nature communications · 2019Article
- Mutations that prevent methylation of cohesin render sensitivity to DNA damage inJournal of cell science · 2018Article
- Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.G3 (Bethesda, Md.) · 2016Article
- The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.PLoS biology · 2014Article
- Synthetic lethality between gene defects affecting a single non-essential molecular pathway with reversible steps.PLoS computational biology · 2013Article
- Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes.Molecular and cellular biology · 2013Article
- Srs2: the "Odd-Job Man" in DNA repair.DNA repair · 2010Review
- Fbh1 limits Rad51-dependent recombination at blocked replication forks.Molecular and cellular biology · 2009Article
- Role of Blm and collaborating factors in recombination and survival following replication stress in Ustilago maydis.DNA repair · 2009Article
- Esc2 and Sgs1 act in functionally distinct branches of the homologous recombination repair pathway in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
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- Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae.Mutation research · 2007Article
- The human F-Box DNA helicase FBH1 faces Saccharomyces cerevisiae Srs2 and postreplication repair pathway roles.Molecular and cellular biology · 2007Article
- Mus81-Eme1-dependent and -independent crossovers form in mitotic cells during double-strand break repair in Schizosaccharomyces pombe.Molecular and cellular biology · 2007Article
- Rqh1 blocks recombination between sister chromatids during double strand break repair, independent of its helicase activity.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- RecQ helicases: lessons from model organisms.Nucleic acids research · 2006Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Our interest in the Schizosaccharomyces pombe RecQ helicase, rqh1+, led us to investigate the function of a related putative DNA helicase, srs2+. We identified the srs2+ homolog in S.pombe, and found that srs2+ is not essential for cell viability. A Deltasrs2 Deltarqh1 double mutant grows extremely slowly with aberrant shaped cells and low viability. This slow growth does not appear to be related to stalled replication, as Deltasrs2 Deltarqh1 cells showed higher survival rates, compared with Deltarqh1, when stalled forks were increased by UV irradiation or hydroxy urea treatment. Consistent with this result, we found that Deltasrs2 Deltarqh1 cells progress through S-phase with a slight delay, but undergo a checkpoint-dependent arrest presumably at G2/M. Further, we found that Deltasrs2 Deltarqh1 slow growth is related to recombination, as loss of either the rhp51+ or rhp57+ recombination genes improves cell growth in the double mutant. Deltasrs2 is also synthetic lethal with Deltarhp54, another homologous recombination gene. This lethality is suppressed in a Deltarhp51 background. Together, these results demonstrate a clear genetic interaction between rqh1+, srs2+ and the genes of the homologous recombination pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.