ArticleNucleic acids research2002
The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 43 citations in OpenAlex.
- Regulation of Pfh1 helicase activity by nucleic acid interactions and mitochondrial SSB.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Homologous recombination repair intermediates promote efficient de novo telomere addition at DNA double-strand breaks.Nucleic acids research · 2020Article
- Pif1 family DNA helicases: A helpmate to RNase H?DNA repair · 2019Review
- TheGenetics · 2019Article
- Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.Nucleic acids research · 2019Article
- Review
- Article
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.Nucleic acids research · 2016Article
- Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding.Nucleic acids research · 2016Article
- Article
- Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.PLoS biology · 2014Article
- Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.G3 (Bethesda, Md.) · 2014Article
- To peep into Pif1 helicase: multifaceted all the way from genome stability to repair-associated DNA synthesis.Journal of microbiology (Seoul, Korea) · 2014Review
- Mapping genomic hotspots of DNA damage by a single-strand-DNA-compatible and strand-specific ChIP-seq method.Genome research · 2013Article
- DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.Genes & development · 2012Article
- The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.Genes & development · 2012Article
- The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis.BMC molecular biology · 2011Article
- Telomeres: structures in need of unwinding.FEBS letters · 2010Review
- TbPIF1, a Trypanosoma brucei mitochondrial DNA helicase, is essential for kinetoplast minicircle replication.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The Cdc24 protein plays an essential role in chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe, most likely via its direct interaction with Dna2, a conserved endonuclease-helicase protein required for Okazaki fragment processing. To gain insights into Cdc24 function, we isolated cold-sensitive chromosomal suppressors of the temperature-sensitive cdc24-M38 allele. One of the complementation groups of such suppressors defined a novel gene, pfh1(+), encoding an 805 amino acid nuclear protein highly homologous to the Saccharomyces cerevisiae Pif1p and Rrm3p DNA helicase family proteins. The purified Pfh1 protein displayed single-stranded DNA-dependent ATPase activity as well as 5' to 3' DNA helicase activity in vitro. Reverse genetic analysis in S.pombe showed that helicase activity was essential for the function of the Pfh1 protein in vivo. Schizosaccharomyces pombe cells carrying the cold-sensitive pfh1-R20 allele underwent cell cycle arrest in late S/G2-phase of the cell cycle when shifted to the restrictive temperature. This arrest was dependent upon the presence of a functional late S/G2 DNA damage checkpoint, suggesting that Pfh1 is required for the completion of DNA replication. Furthermore, at their permissive temperature pfh1-R20 cells were highly sensitive to the DNA-alkylating agent methyl methanesulphonate, implying a further role for Pfh1 in the repair of DNA damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.