ArticleNucleic acids research2002
Translational fusions with the engrailed repressor domain efficiently convert plant transcription factors into dominant-negative functions.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Temporal neuronal differentiation programs safeguard neuronal diversity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Chimeric NANOG repressors inhibit glioblastoma growth in vivo in a context-dependent manner.Scientific reports · 2019Article
- Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress.International journal of molecular sciences · 2018Review
- Antagonistic roles for KNOX1 and KNOX2 genes in patterning the land plant body plan following an ancient gene duplication.PLoS genetics · 2015Article
- Genome-wide analysis reveals gene expression and metabolic network dynamics during embryo development in Arabidopsis.Plant physiology · 2011Article
- Stage-specific regulation of Solanum lycopersicum leaf maturation by class 1 KNOTTED1-LIKE HOMEOBOX proteins.The Plant cell · 2009Article
- Article
- KNOX lost the OX: the Arabidopsis KNATM gene defines a novel class of KNOX transcriptional regulators missing the homeodomain.The Plant cell · 2008Article
- Article
- Legume transcription factors: global regulators of plant development and response to the environment.Plant physiology · 2007Review
- Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana.Nucleic acids research · 2006Article
- Engrailed-ZmOCL1 fusions cause a transient reduction of kernel size in maize.Plant molecular biology · 2005Article
- Aux/IAA proteins contain a potent transcriptional repression domain.The Plant cell · 2004Article
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3 authors.
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Abstract
Evidence is provided that plant transcription factors can be efficiently reprogrammed to dominant- negative functions by the use of a repressor domain of the engrailed (en) gene from Drosophila. Ectopic expression of translational fusions between the en(298) N-terminus and the complete coding regions of the SHOOTMERISTEMLESS, APETALA3, PISTILLATA and KNAT1 transcription factors results in trans-dominant functions which phenocopy loss-of-function mutants. The combination of the dominant-negative en(298)-STM function with the hormone-binding domain of the glucocorticoid receptor provides strong evidence that phenocopies rely on the incorporation of the chimeric protein into the nuclear compartment. By this dominant-negative approach KNAT1 was rapidly identified to be encoded by the BREVIPEDICELLUS locus. Dominant-negative chimeric proteins may be of wide use to elucidate biological functions of plant transcriptional activators and may be suitable to study protein-protein interactions in planta.
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