ArticleNucleic acids research2002
Domains of human U4atac snRNA required for U12-dependent splicing in vivo.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Phenotypic spectrum of RNU4ATAC-related spliceosomopathies: four novel cases and integrated reevaluation of previously reported patients.Orphanet journal of rare diseases · 2026Article
- Transcriptome-wide outlier approach identifies individuals with minor spliceopathies.American journal of human genetics · 2025Article
- Mutations in the non-coding RNU4ATAC gene affect the homeostasis and function of the Integrator complex.Nucleic acids research · 2023Article
- Disruption of exon-bridging interactions between the minor and major spliceosomes results in alternative splicing around minor introns.Nucleic acids research · 2021Article
- Clinical interpretation of variants identified in RNU4ATAC, a non-coding spliceosomal gene.PloS one · 2020Article
- Extending the critical regions for mutations in the non-coding geneClinical case reports · 2018Article
- U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.Scientific reports · 2016Article
- Two novel mutations in RNU4ATAC in two siblings with an atypical mild phenotype of microcephalic osteodysplastic primordial dwarfism type 1.Clinical dysmorphology · 2016Article
- Compound heterozygous mutations in the noncoding RNU4ATAC cause Roifman Syndrome by disrupting minor intron splicing.Nature communications · 2015Article
- Biochemical defects in minor spliceosome function in the developmental disorder MOPD I.RNA (New York, N.Y.) · 2014Article
- In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop.RNA (New York, N.Y.) · 2012Article
- Functionally important structural elements of U12 snRNA.Nucleic acids research · 2011Article
- Mutations in U4atac snRNA, a component of the minor spliceosome, in the developmental disorder MOPD I.Science (New York, N.Y.) · 2011Article
- The conserved 3' end domain of U6atac snRNA can direct U6 snRNA to the minor spliceosome.RNA (New York, N.Y.) · 2009Article
- Evolution of spliceosomal snRNA genes in metazoan animals.Journal of molecular evolution · 2008Article
- Free energy landscapes of RNA/RNA complexes: with applications to snRNA complexes in spliceosomes.Journal of molecular biology · 2006Article
- Recycling of the U12-type spliceosome requires p110, a component of the U6atac snRNP.Molecular and cellular biology · 2004Article
- U4 small nuclear RNA can function in both the major and minor spliceosomes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing.Genome biology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
U4atac snRNA forms a base-paired complex with U6atac snRNA. Both snRNAs are required for the splicing of the minor U12-dependent class of eukaryotic nuclear introns. We have developed a new genetic suppression assay to investigate the in vivo roles of several regions of U4atac snRNA in U12-dependent splicing. We show that both the stem I and stem II regions, which have been proposed to pair with U6atac snRNA, are required for in vivo splicing. Splicing activity also requires U4atac sequences in the 5' stem-loop element that bind a 15.5 kDa protein that also binds to a similar region of U4 snRNA. In contrast, mutations in the region immediately following the stem I interaction region, as well as a deletion of the distal portion of the 3' stem-loop element, were active for splicing. Complete deletion of the 3' stem-loop element abolished in vivo splicing function as did a mutation of the Sm protein binding site. These results show that the in vivo sequence requirements of U4atac snRNA are similar to those described previously for U4 snRNA using in vitro assays and provide experimental support for models of the U4atac/U6atac snRNA interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.