ArticleJournal of clinical microbiology2002
Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 91 citations in OpenAlex.
- Prevalence and Characteristics ofMicroorganisms · 2026Article
- The Landscape of Salmonella enterica Serovar Gallinarum-Pullorum Antimicrobial Resistance in Bangladesh's Poultry Industry: A Combined Phenotypic and Molecular Study.MicrobiologyOpen · 2026Article
- Intracellular Experimental Evolution ofACS infectious diseases · 2023Article
- Antibiotic Resistance in Non-TyphoidalPathogens (Basel, Switzerland) · 2022Article
- High Genetic Diversity and Antimicrobial Resistance inMicroorganisms · 2022Article
- Identification and Characterization of Salmonella enterica Serotype Newport Isolates with Decreased Susceptibility to Ciprofloxacin in the United States.Antimicrobial agents and chemotherapy · 2018Article
- Prevalence of plasmid-mediated quinolone resistance in Escherichia coli isolated from diseased animals in Taiwan.The Journal of veterinary medical science · 2017Article
- Using In Vitro Dynamic Models To Evaluate Fluoroquinolone Activity against Emergence of Resistant Salmonella enterica Serovar Typhimurium.Antimicrobial agents and chemotherapy · 2017Article
- Article
- Rapid Detection of Genomic Mutations in gyrA and parC Genes of Escherichia coli by Multiplex Allele Specific Polymerase Chain Reaction.Journal of clinical laboratory analysis · 2016Article
- Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.Genome biology and evolution · 2015Article
- Identification and characterization of multidrug-resistant Salmonella enterica serotype Albert isolates in the United States.Antimicrobial agents and chemotherapy · 2015Article
- Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum.Scientific reports · 2014Article
- Contribution of phenotypic heterogeneity to adaptive antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Influence of Sterilized Human Fecal Extract on the Sensitivity of Salmonella enterica ATCC 13076 and Listeria monocytogenes ATCC 15313 to Enrofloxacin.Antibiotics (Basel, Switzerland) · 2013Article
- Amino acid substitutions in gyrA and parC associated with quinolone resistance in nalidixic acid-resistant Salmonella isolates.Irish veterinary journal · 2013Article
- Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.Osong public health and research perspectives · 2012Article
- Effect of sterilized human fecal extract on the sensitivity of Escherichia coli ATCC 25922 to enrofloxacin.The Journal of antibiotics · 2012Article
- A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A.The Journal of antimicrobial chemotherapy · 2010Article
- Mechanisms of resistance in nontyphoidal Salmonella enterica strains exhibiting a nonclassical quinolone resistance phenotype.Antimicrobial agents and chemotherapy · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a rapid screening and identification method for DNA sequence variation detection in the quinolone resistance-determining region of gyrA from Salmonella serovars. A total of 203 isolates of Salmonella were screened using this method. DHPLC analysis of 14 isolates representing each type of novel or multiple mutations and the wild type were compared with LightCycler-based PCR-gyrA hybridization mutation assay (GAMA) and single-strand conformational polymorphism (SSCP) analyses. The 14 isolates gave seven different SSCP patterns, and LightCycler detected four different mutations. DHPLC detected 11 DNA sequence variants at eight different codons, including those detected by LightCycler or SSCP. One of these mutations was silent. Five isolates contained multiple mutations, and four of these could be distinguished from the composite sequence variants by their DHPLC profile. Seven novel mutations were identified at five different loci not previously described in quinolone-resistant salmonella. DHPLC analysis proved advantageous for the detection of novel and multiple mutations. DHPLC also provides a rapid, high-throughput alternative to LightCycler and SSCP for screening frequently occurring mutations.
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