ArticleJournal of clinical microbiology2002
Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Serological screening for cytomegalovirus in a leucodepleted blood supply: A systematic review.Vox sanguinis · 2026Pooled it
- Inter-laboratory variability in cytomegalovirus DNA quantification: implications for standardization and clinical monitoring.Journal of clinical microbiology · 2025Article
- Quantification of low-level human cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR.Journal of virological methods · 2024Article
- Metagenomic search of viral coinfections in herpes simplex encephalitis patients.Journal of neurovirology · 2023Article
- Direct Nanopore Sequencing of Human Cytomegalovirus Genomes from High-Viral-Load Clinical Samples.Viruses · 2023Article
- Whole Blood versus Plasma Samples-How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia?Pathogens (Basel, Switzerland) · 2022Article
- The Standardization and Control of Serology and Nucleic Acid Testing for Infectious Diseases.Clinical microbiology reviews · 2021Review
- Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo.Medicina (Kaunas, Lithuania) · 2021Article
- Role of Envelope Glycoprotein Complexes in Cell-Associated Spread of Human Cytomegalovirus.Viruses · 2021Article
- Next-generation sequencing in the diagnosis of viral encephalitis: sensitivity and clinical limitations.Scientific reports · 2020Article
- Where do we Stand after Decades of Studying Human Cytomegalovirus?Microorganisms · 2020Review
- Viral Metagenomics on Cerebrospinal Fluid.Genes · 2019Article
- Does Size Matter? Comparison of Extraction Yields for Different-Sized DNA Fragments by Seven Different Routine and Four New Circulating Cell-Free Extraction Methods.Journal of clinical microbiology · 2018Article
- Review
- Determination of the Biological Form of Human Cytomegalovirus DNA in the Plasma of Solid-Organ Transplant Recipients.The Journal of infectious diseases · 2017Article
- Sequence Variation in Amplification Target Genes and Standards Influences Interlaboratory Comparison of BK Virus DNA Load Measurement.Journal of clinical microbiology · 2015Article
- Commutability of the First World Health Organization International Standard for Human Cytomegalovirus.Journal of clinical microbiology · 2015Article
- Cytomegalovirus DNA stability in EDTA anti-coagulated whole blood and plasma samples.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma Cytomegalovirus DNAemia in allogeneic stem cell transplant recipients.Journal of clinical microbiology · 2011Article
- Viral etiology of encephalitis in children in southern Vietnam: results of a one-year prospective descriptive study.PLoS neglected tropical diseases · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitation of cytomegalovirus (CMV) DNA in plasma and serum by PCR is increasingly used to identify patients at risk for developing CMV disease and to monitor the efficacy of antiviral therapy. Although CMV DNA levels are generally interpreted as viral loads, the exact nature of the viral DNA in these specimens is unknown. We studied the state of CMV DNA in plasma and serum specimens obtained from three renal transplant recipients at peak viral DNA levels during primary CMV infection. For this purpose, DNA isolated from these specimens was fractionated by size, and CMV DNA levels in the resulting DNA fractions were measured by quantitative PCR targeted at large (578-bp) and small (134-bp) amplicons. These experiments showed that the molecular sizes of DNA fragments from which CMV DNA is amplified were small (<2,000 bp), indicating that CMV DNA in plasma and serum is highly fragmented. Furthermore, CMV DNA levels were consistently higher when targeted at the smaller amplicon, providing additional evidence for the fragmentation of viral DNA. In conclusion, the first results with three patients have shown that CMV DNA in plasma and serum is highly fragmented and does not necessarily reflect the amount of infectious virus. These observations have potential consequences for understanding CMV pathogenesis and interpreting CMV DNA levels in individual patient management.
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