ArticleGene therapy2002
Possible mechanism of gene transfer into early to mid-gestational mouse fetuses by tail vein injection.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Recent Genome-Editing Approaches toward Post-Implanted Fetuses in Mice.Biotech (Basel (Switzerland)) · 2023Review
- Development of Novel Heparin/Protamine Nanoparticles Useful for Delivery of Exogenous Proteins In Vitro and In Vivo.Nanomaterials (Basel, Switzerland) · 2020Article
- Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome.Cells · 2020Article
- Review
- Transplacental Gene Delivery (TPGD) as a Noninvasive Tool for Fetal Gene Manipulation in Mice.International journal of molecular sciences · 2019Review
- Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.Biology direct · 2016Review
- Maternal pravastatin prevents altered fetal brain development in a preeclamptic CD-1 mouse model.PloS one · 2014Article
- Kidney-specific expression of GFP by in-utero delivery of pseudotyped adeno-associated virus 9.Molecular therapy. Methods & clinical development · 2014Article
- Genetic manipulation of the mouse developing hypothalamus through in utero electroporation.Journal of visualized experiments : JoVE · 2013Article
- Effective silencing of Sry gene with RNA interference in developing mouse embryos resulted in feminization of XY gonad.Journal of biomedicine & biotechnology · 2012Article
- In vivo gene delivery by cationic tetraamino fullerene.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Transplacental RNAi: deciphering gene function in the postimplantation-staged embryo.Journal of biomedicine & biotechnology · 2006Article
- A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid.BMC nephrology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our aim is to develop a simple gene transfer method into egg cylinder and mid-gestational murine embryos. We examined whether plasmid/lipid complexes injected into the tail veins of pregnant transgenic mice can be transferred to fetuses at E 4.5-13.5. When pregnant CETZ-17 mice carrying a transgene consisting of a ubiquitous promoter, floxed EGFP/CAT and the LacZ gene, were injected with a Cre expression vector DNA/lipid complex, Cre-mediated excision of the transgenes, as evaluated by X-gal staining, occurred in 10-50% of fetuses treated at E 11.5-13.5. Although younger embryos remained unstained, PCR analysis revealed low levels of the Cre vector DNA and recombined transgene. To examine the fate of a solution given intravenously, we injected trypan blue or fluorescence-labeled plasmid DNA/lipid complexes into females at E 5.5-11.5 and E 6.5, respectively. Both collected in the visceral endoderm (VE) lineage, but were undetectable in the embryo proper. These findings suggest that substances in maternal blood are delivered to post-implantation embryos via cells of the VE lineage and placenta, but that most are trapped in the VE. If significantly improved, gene transfer to fetuses by injection into the maternal circulation may become a promising tool in fetal gene therapy and embryological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.