ArticleGene therapy2002
Inhibition of cartilage destruction by double gene transfer of IL-1Ra and IL-10 involves the activin pathway.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Visfatin/pre-B-cell colony-enhancing factor (PBEF), a proinflammatory and cell motility-changing factor in rheumatoid arthritis.The Journal of biological chemistry · 2012Trial
- Orthobiologics in knee osteoarthritis, dream or reality?Archives of orthopaedic and trauma surgery · 2024Review
- Gene therapy approaches for equine osteoarthritis.Frontiers in veterinary science · 2022Review
- Genetics in Cartilage Lesions: Basic Science and Therapy Approaches.International journal of molecular sciences · 2020Review
- The activin-follistatin anti-inflammatory cycle is deregulated in synovial fibroblasts.Arthritis research & therapy · 2019Article
- Targeting Activated Synovial Fibroblasts in Rheumatoid Arthritis by Peficitinib.Frontiers in immunology · 2019Article
- Gene Delivery to Joints by Intra-Articular Injection.Human gene therapy · 2018Review
- Gene therapy approaches to regenerating the musculoskeletal system.Nature reviews. Rheumatology · 2015Review
- Interleukin-17 (IL-17) and IL-1 activate translation of overlapping sets of mRNAs, including that of the negative regulator of inflammation, MCPIP1.The Journal of biological chemistry · 2013Article
- Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts.Arthritis research & therapy · 2010Article
- Synovial fibroblasts spread rheumatoid arthritis to unaffected joints.Nature medicine · 2009Article
- Concepts in gene therapy for cartilage repair.Injury · 2008Review
- Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells.Digestive diseases and sciences · 2007Article
- Activin A suppresses interleukin-1-induced matrix metalloproteinase 3 secretion in human chondrosarcoma cells.Rheumatology international · 2007Article
- Cells of the synovium in rheumatoid arthritis. Chondrocytes.Arthritis research & therapy · 2007Review
- Direct adenovirus-mediated IGF-I gene transduction of synovium induces persisting synovial fluid IGF-I ligand elevations.Gene therapy · 2006Article
- [Role of gene therapy in trauma and orthopedic surgery].Der Unfallchirurg · 2006Review
- A preliminary in vitro study into the use of IL-1Ra gene therapy for the inhibition of intervertebral disc degeneration.International journal of experimental pathology · 2006Article
- Regulated and constitutive expression of anti-inflammatory cytokines by nontransforming herpesvirus saimiri vectors.Gene therapy · 2005Article
- Targeting cathepsin L (CL) by specific ribozymes decreases CL protein synthesis and cartilage destruction in rheumatoid arthritis.Gene therapy · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of the study was to determine the effects and the molecular background of interleukin-1 receptor antagonist (IL-1Ra) and vIL-10 double gene transfer into human synovial fibroblasts from patients with rheumatoid arthritis (RA) using the SCID mouse model for cartilage erosion in RA. RA synovial fibroblasts were transduced with retro- or adenoviruses encoding IL-1Ra and/or viral IL-10 (vIL-10). SCID mice were engrafted subcutaneously with IL-1Ra and vIL-10 transduced human rheumatoid synovial fibroblasts and normal cartilage. In parallel, gene expression analysis before and after gene transfer using RNA arbitrarily primed PCR in combination with cDNA array was performed. vIL-10 and IL-1Ra double gene transfer resulted in inhibition of cartilage invasion and degradation by RA synovial fibroblasts when compared with control transduced and non-transduced implants. Expression of key genes that were altered after double gene transfer were related to the activin pathway. The results demonstrate not only that virus-based gene transfer using a combination of two joint-protective genes is a feasible approach to inhibit cartilage degradation by activated RA synovial fibroblasts, but also that the underlying molecular effects include modulation of the activin pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.