ArticleProceedings of the National Academy of Sciences of the United States of America2002
The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 50 citations in OpenAlex.
- Ribosomal RNA Transcription Machineries in Intestinal Protozoan Parasites: A Bioinformatic Analysis.Acta parasitologica · 2022Article
- A cell-based screening system for RNA polymerase I inhibitors.MedChemComm · 2019Article
- Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit.Nucleic acids research · 2015Article
- Review
- Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.Biochemistry · 2012Article
- Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7.Molecular and biochemical parasitology · 2011Article
- RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei.EMBO reports · 2009Article
- Genomewide recruitment analysis of Rpb4, a subunit of polymerase II in Saccharomyces cerevisiae, reveals its involvement in transcription elongation.Eukaryotic cell · 2008Article
- Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Site specific phosphorylation of yeast RNA polymerase I.Nucleic acids research · 2008Article
- Deletion of Rnt1p alters the proportion of open versus closed rRNA gene repeats in yeast.Molecular and cellular biology · 2008Article
- Active RNA polymerase I of Trypanosoma brucei harbors a novel subunit essential for transcription.Molecular and cellular biology · 2007Article
- Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.Nucleic acids research · 2007Article
- RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor.Molecular and cellular biology · 2006Article
- Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III.Nucleic acids research · 2006Article
- The RNA polymerase I transcription machinery.Biochemical Society symposium · 2006Review
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Nucleic acids research · 2004Article
- Structural and functional homology between the RNAP(I) subunits A14/A43 and the archaeal RNAP subunits E/F.Nucleic acids research · 2003Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription. The pol I-Rrn3 complex is the only form of enzyme competent for promoter-dependent transcription initiation. In this paper, using biochemical and genetic approaches, we demonstrate that the A43 polypeptide forms a stable heterodimer with the A14 pol I subunit and interacts with the common ABC23 subunit, the yeast counterpart of the omega subunit of bacterial RNA polymerase. We show by immunoelectronic microscopy that A43, ABC23, and A14 colocalize in the three-dimensional structure of the pol I, and we demonstrate that the presence of A43 is required for the stabilization of both A14 and ABC23 within the pol I. Because the N-terminal half of A43 is clearly related to the pol II Rpb7 subunit, we propose that the A43-A14 pair is likely the pol I counterpart of the Rpb7-Rpb4 heterodimer, although A14 distinguishes from Rpb4 by specific sequence and structure features. This hypothesis, combined with our structural data, suggests a new localization of Rpb7-Rpb4 subunits in the three-dimensional structure of yeast pol II.
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