ArticleApplied and environmental microbiology2002
Isolation and functional analysis of a gene, tcsB, encoding a transmembrane hybrid-type histidine kinase from Aspergillus nidulans.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 78 citations in OpenAlex.
- Biocontrol Efficacy and Mechanism of Action ofJournal of fungi (Basel, Switzerland) · 2026Article
- Hybrid histidine kinases shape the architecture of the high osmolarity glycerol (HOG) pathway: from Saccharomyces cerevisiae to the priority pathogen Aspergillus fumigatus and other filamentous fungi.FEMS microbiology reviews · 2026Review
- Hydrophobins inFrontiers in fungal biology · 2025Article
- Regulation of Fungal Morphogenesis and Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2023Article
- MaSln1, a Conserved Histidine Protein Kinase, Contributes to Conidiation Pattern Shift Independent of the MAPK Pathway inMicrobiology spectrum · 2022Article
- The putative sensor histidine kinase VadJ coordinates development and sterigmatocystin production in Aspergillus nidulans.Journal of microbiology (Seoul, Korea) · 2021Article
- Histidine Kinase Sln1 and cAMP/PKA Signaling Pathways Antagonistically RegulateJournal of fungi (Basel, Switzerland) · 2021Article
- Downregulation of theFrontiers in fungal biology · 2021Article
- The Sensor Proteins BcSho1 and BcSln1 Are Involved in, Though Not Essential to, Vegetative Differentiation, Pathogenicity and Osmotic Stress Tolerance inFrontiers in microbiology · 2019Article
- Functional characterization of the upstream components of the Hog1-like kinase cascade in hyperosmotic and carbon sensing inBiotechnology for biofuels · 2018Article
- Fungal Histidine Phosphotransferase Plays a Crucial Role in Photomorphogenesis and Pathogenesis inFrontiers in chemistry · 2017Article
- Osmosensing and osmoregulation in unicellular eukaryotes.World journal of microbiology & biotechnology · 2015Review
- Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions.FEBS open bio · 2014Article
- Current understanding of HOG-MAPK pathway in Aspergillus fumigatus.Mycopathologia · 2013Review
- Receptor-mediated signaling in Aspergillus fumigatus.Frontiers in microbiology · 2013Article
- Article
- HOG-MAPK signaling regulates the adaptive responses of Aspergillus fumigatus to thermal stress and other related stress.Mycopathologia · 2012Article
- Characterization of the conserved phosphorylation site in the Aspergillus nidulans response regulator SrrA.Current genetics · 2011Article
- A two-component histidine kinase, MoSLN1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae.Current genetics · 2010Article
- Role of the osmotic stress regulatory pathway in morphogenesis and secondary metabolism in filamentous fungi.Toxins · 2010Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We cloned and characterized a novel Aspergillus nidulans histidine kinase gene, tcsB, encoding a membrane-type two-component signaling protein homologous to the yeast osmosensor synthetic lethal N-end rule protein 1 (SLN1), which transmits signals through the high-osmolarity glycerol response 1 (HOG1) mitogen-activated protein kinase (MAPK) cascade in yeast cells in response to environmental osmotic stimuli. From an A. nidulans cDNA library, we isolated a positive clone containing a 3,210-bp open reading frame that encoded a putative protein consisting of 1,070 amino acids. The predicted tcsB protein (TcsB) has two probable transmembrane regions in its N-terminal half and has a high degree of structural similarity to yeast Sln1p, a transmembrane hybrid-type histidine kinase. Overexpression of the tcsB cDNA suppressed the lethality of a temperature-sensitive osmosensing-defective sln1-ts yeast mutant. However, tcsB cDNAs in which the conserved phosphorylation site His(552) residue or the phosphorelay site Asp(989) residue had been replaced failed to complement the sln1-ts mutant. In addition, introduction of the tcsB cDNA into an sln1delta sho1delta yeast double mutant, which lacked two osmosensors, suppressed lethality in high-salinity media and activated the HOG1 MAPK. These results imply that TcsB functions as an osmosensor histidine kinase. We constructed an A. nidulans strain lacking the tcsB gene (tcsBdelta) and examined its phenotype. However, unexpectedly, the tcsBdelta strain did not exhibit a detectable phenotype for either hyphal development or morphology on standard or stress media. Our results suggest that A. nidulans has more complex and robust osmoregulatory systems than the yeast SLN1-HOG1 MAPK cascade.
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