ArticleThe Journal of cell biology2002
Visualization of replication initiation and elongation in Drosophila.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Finishing the egg.Genetics · 2024Article
- The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo.PLoS genetics · 2023Article
- Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication inMicroorganisms · 2023Article
- Review
- Article
- Rapid DNA Synthesis During EarlyGenetics · 2017Article
- DNA Replication Control DuringGenetics · 2017Review
- Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- The Histone Variant H3.3 Is Enriched at Drosophila Amplicon Origins but Does Not Mark Them for Activation.G3 (Bethesda, Md.) · 2016Article
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Replication fork progression during re-replication requires the DNA damage checkpoint and double-strand break repair.Current biology : CB · 2015Article
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- DNA copy-number control through inhibition of replication fork progression.Cell reports · 2014Article
- The role of transcription in the activation of a Drosophila amplification origin.G3 (Bethesda, Md.) · 2014Article
- Incomplete replication generates somatic DNA alterations within Drosophila polytene salivary gland cells.Genes & development · 2014Article
- Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity.The Plant cell · 2012Article
- Developmental control of gene copy number by repression of replication initiation and fork progression.Genome research · 2012Article
- Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Chorion gene amplification in the ovaries of Drosophila melanogaster is a powerful system for the study of metazoan DNA replication in vivo. Using a combination of high-resolution confocal and deconvolution microscopy and quantitative realtime PCR, we found that initiation and elongation occur during separate developmental stages, thus permitting analysis of these two phases of replication in vivo. Bromodeoxyuridine, origin recognition complex, and the elongation factors minichromosome maintenance proteins (MCM)2-7 and proliferating cell nuclear antigen were precisely localized, and the DNA copy number along the third chromosome chorion amplicon was quantified during multiple developmental stages. These studies revealed that initiation takes place during stages 10B and 11 of egg chamber development, whereas only elongation of existing replication forks occurs during egg chamber stages 12 and 13. The ability to distinguish initiation from elongation makes this an outstanding model to decipher the roles of various replication factors during metazoan DNA replication. We utilized this system to demonstrate that the pre-replication complex component, double-parked protein/cell division cycle 10-dependent transcript 1, is not only necessary for proper MCM2-7 localization, but, unexpectedly, is present during elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.