ArticleBMC neuroscience2002
Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury.
Article in BMC neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 255 papers.
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255 citing papers in PubMed, 558 citations in OpenAlex.
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- Similarities and differences in the response and molecular characteristics of peripheral sensory neurons associated with pain and itch.Acta biochimica et biophysica Sinica · 2025Article
- Pregabalin produces analgesia in males but not females in an animal model of chronic widespread muscle pain.Pain reports · 2024Article
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- Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling.Molecular neurobiology · 2023Article
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- Enhanced axonal regeneration of ALS patient iPSC-derived motor neurons harboring SOD1Scientific reports · 2023Article
- Phylogenetic Analysis Provides Insight Into the Molecular Evolution of Nociception and Pain-Related Proteins.Evolutionary bioinformatics online · 2023Article
- RSK1 promotes mammalian axon regeneration by inducing the synthesis of regeneration-related proteins.PLoS biology · 2022Article
- The Jun-dependent axon regeneration gene program: Jun promotes regeneration over plasticity.Human molecular genetics · 2022Article
- SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.Journal of nanobiotechnology · 2022Article
- The involvement of immune system in intervertebral disc herniation and degeneration.JOR spine · 2022Review
- Beyond CGRP: The calcitonin peptide family as targets for migraine and pain.British journal of pharmacology · 2022Review
- Gadd45 in Neuronal Development, Function, and Injury.Advances in experimental medicine and biology · 2022Review
- Bibliometric analysis of research on gene expression in spinal cord injury.Frontiers in molecular neuroscience · 2022Article
- Calcium homeostasis in parvalbumin DRG neurons is altered after sciatic nerve crush and sciatic nerve transection injuries.Journal of neurophysiology · 2021Article
195 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundRat oligonucleotide microarrays were used to detect changes in gene expression in the dorsal root ganglion (DRG) 3 days following sciatic nerve transection (axotomy). Two comparisons were made using two sets of triplicate microarrays, naïve versus naïve and naïve versus axotomy.
resultsMicroarray variability was assessed using the naïve versus naïve comparison. These results support use of a P < 0.05 significance threshold for detecting regulated genes, despite the large number of hypothesis tests required. For the naïve versus axotomy comparison, a 2-fold cut off alone led to an estimated error rate of 16%; combining a >1.5-fold expression change and P < 0.05 significance reduced the estimated error to 5%. The 2-fold cut off identified 178 genes while the combined >1.5-fold and P < 0.05 criteria generated 240 putatively regulated genes, which we have listed. Many of these have not been described as regulated in the DRG by axotomy. Northern blot, quantitative slot blots and in situ hybridization verified the expression of 24 transcripts. These data showed an 83% concordance rate with the arrays; most mismatches represent genes with low expression levels reflecting limits of array sensitivity. A significant correlation was found between actual mRNA differences and relative changes between microarrays (r2 = 0.8567). Temporal patterns of individual genes regulation varied.
conclusionsWe identify parameters for microarray analysis which reduce error while identifying many putatively regulated genes. Functional classification of these genes suggest reorganization of cell structural components, activation of genes expressed by immune and inflammatory cells and down-regulation of genes involved in neurotransmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.