ArticleThe Biochemical journal2003
Role of cdc2 kinase phosphorylation and conserved N-terminal proteolysis motifs in cytoplasmic polyadenylation-element-binding protein (CPEB) complex dissociation and degradation.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Phosphoproteomic identification of Mos-MAPK targets in meiotic cell cycle and asymmetric oocyte divisions.The Journal of cell biology · 2025Article
- MAPK signaling couples SCF-mediated degradation of translational regulators to oocyte meiotic progression.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structural Analysis of the Pin1-CPEB1 interaction and its potential role in CPEB1 degradation.Scientific reports · 2015Article
- A role of CPEB1 in the modulation of proliferation and neuronal maturation of rat primary neural progenitor cells.Neurochemical research · 2013Article
- Multiple binding of repressed mRNAs by the P-body protein Rck/p54.RNA (New York, N.Y.) · 2012Article
- To polyadenylate or to deadenylate: that is the question.Cell cycle (Georgetown, Tex.) · 2010Review
- Distinct functions of maternal and somatic Pat1 protein paralogs.RNA (New York, N.Y.) · 2010Article
- Cytoplasmic polyadenylation and cytoplasmic polyadenylation element-dependent mRNA regulation are involved in Xenopus retinal axon development.Neural development · 2009Article
- Mechanism of degradation of CPEB during Xenopus oocyte maturation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.Molecular and cellular biology · 2005Article
- Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic polyadenylation-element-binding protein (CPEB) is a well-characterized and important regulator of translation of maternal mRNA in early development in organisms ranging from worms, flies and clams to frogs and mice. Previous studies provided evidence that clam and Xenopus CPEB are hyperphosphorylated at germinal vesicle breakdown (GVBD) by cdc2 kinase, and degraded shortly after. To examine the conserved features of CPEB that mediate its modification during meiotic maturation, we microinjected mRNA encoding wild-type and mutated clam CPEB into Xenopus oocytes that were subsequently allowed to mature with progesterone. We observed that (i) ectopically expressed clam CPEB is phosphorylated at GVBD and subsequently degraded, mirroring the fate of the endogenous Xenopus CPEB protein, (ii) mutation of nine Ser/Thr Pro-directed kinase sites prevents phosphorylation and degradation and (iii) deletion of the PEST box, and to a lesser extent of the putative cyclin destruction box, generates a stable and phosphorylated version of CPEB. We conclude that phosphorylation of both consensus and non-consensus sites by cdc2 kinase targets clam CPEB for PEST-mediated destruction. We also show that phosphorylation of CPEB mediates its dissociation from ribonucleoprotein complexes, prior to degradation. Our findings reinforce results obtained in Xenopus, and have implications for CPEB from other invertebrates including Drosophila, Caenorhabditis elegans and Aplysia, which lack PEST boxes.
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