ArticleJournal of bacteriology2002
Poly-beta-hydroxybutyrate biosynthesis in the facultative methylotroph methylobacterium extorquens AM1: identification and mutation of gap11, gap20, and phaR.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose.Microbial cell factories · 2026Article
- Pregnenolone Bioproduction in Engineered Methylobacteria: Design and Elaboration.International journal of molecular sciences · 2025Article
- Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges.Advances in biochemical engineering/biotechnology · 2025Review
- Biosynthesis of polyhydroxybutyrate byFrontiers in plant science · 2024Article
- Article
- Construction of a Rhodobacter sphaeroides Strain That Efficiently Produces Hydrogen Gas from Acetate without Poly(β-Hydroxybutyrate) Accumulation: Insight into the Role of PhaR in Acetate Metabolism.Applied and environmental microbiology · 2022Article
- Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7.AMB Express · 2019Article
- Designing and EngineeringFrontiers in microbiology · 2019Article
- Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation.BMC microbiology · 2018Article
- The Opportunity for High-Performance Biomaterials from Methane.Microorganisms · 2016Review
- Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022.Microbial cell factories · 2015Article
- Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp.BioMed research international · 2015Review
- Inter individual variations of the fish skin microbiota: host genetics basis of mutualism?PloS one · 2014Article
- The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate.The Journal of biological chemistry · 2012Article
- The diversity of endophytic methylotrophic bacteria in an oil-contaminated and an oil-free mangrove ecosystem and their tolerance to heavy metals.Biotechnology research international · 2012Article
- Article
- Methanol assimilation in Methylobacterium extorquens AM1: demonstration of all enzymes and their regulation.PloS one · 2010Article
- Alternative route for glyoxylate consumption during growth on two-carbon compounds by Methylobacterium extorquens AM1.Journal of bacteriology · 2010Article
- Population heterogeneity in Methylobacterium extorquens AM1.Microbiology (Reading, England) · 2009Article
- Demonstration of the ethylmalonyl-CoA pathway by using 13C metabolomics.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Methylobacterium extorquens AM1, a serine cycle facultative methylotroph, accumulates poly-beta-hydroxybutyrate (PHB) as a carbon and energy reserve material during growth on both multicarbon- and single-carbon substrates. Recently, the identification and mutation of the genes involved in the biosynthesis and degradation of PHB have been described for this bacterium, demonstrating that two of the genes of the PHB cycle (phaA and phaB) are also involved in C(1) and C(2) metabolism, as part of a novel pathway for glyoxylate regeneration in the serine cycle (N. Korotkova and M. E. Lidstrom, J. Bacteriol. 183:1038-1046, 2001; N. Korotkova, L. Chistoserdova, V. Kuksa, and M. E. Lidstrom, J. Bacteriol. 184:1750-1758, 2002). In this work, three new genes involved in PHB biosynthesis in this bacterium have been investigated via mutation and phenotypic analysis: gap11, gap20, and phaR. We demonstrate that gap11 and gap20 encode two major granule-associated proteins (phasins) and that mutants with mutations in these genes are defective in PHB production and also in growth on C(2) compounds, while they show wild-type growth characteristics on C(1) or multicarbon compounds. The phaR mutant shows defects in both PHB accumulation and growth characteristics when grown on C(1) compounds and has defects in PHB accumulation but grows normally on C(3) and C(4) compounds, while both PHB accumulation and growth rate are at wild-type levels during growth on C(2) compounds. Our results suggest that this phenotype is due to altered fluxes of acetyl coenzyme A (CoA), a major intermediate in C(1), C(2), and heterotrophic metabolism in M. extorquens AM1, as well as the entry metabolite for the PHB cycle. Therefore, it seems likely that PhaR acts to control acetyl-CoA flux to PHB in this methylotrophic bacterium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.