ArticleGenetics2002
A unique class of conditional sir2 mutants displays distinct silencing defects in Saccharomyces cerevisiae.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Physical interactions within the SIR heterochromatin complex potentiate inter-subunit communication and gene repression.Cell reports · 2026Article
- Non-canonical chromatin-based functions for the threonine metabolic pathway.Scientific reports · 2024Article
- Article
- Functions for diverse metabolic activities in heterochromatin.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Sumoylation of Sir2 differentially regulates transcriptional silencing in yeast.Nucleic acids research · 2015Article
- A moonlighting metabolic protein influences repair at DNA double-stranded breaks.Nucleic acids research · 2015Article
- The SAGA histone deubiquitinase module controls yeast replicative lifespan via Sir2 interaction.Cell reports · 2014Article
- Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding.Genes & development · 2013Article
- The role of multiple marks in epigenetic silencing and the emergence of a stable bivalent chromatin state.PLoS computational biology · 2013Article
- Suppression analysis of esa1 mutants in Saccharomyces cerevisiae links NAB3 to transcriptional silencing and nucleolar functions.G3 (Bethesda, Md.) · 2012Article
- Natural genetic variation in yeast longevity.Genome research · 2012Article
- Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae.The Journal of biological chemistry · 2010Article
- Co-infection with pansensitive and multidrug-resistant strains of Mycobacterium tuberculosis.Emerging infectious diseases · 2009Article
- Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2.Molecular and cellular biology · 2008Article
- Nuclear export modulates the cytoplasmic Sir2 homologue Hst2.EMBO reports · 2006Article
- A telomeric repeat sequence adjacent to a DNA double-stranded break produces an anticheckpoint.Genes & development · 2005Article
- Article
- Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silencing provides a critical means of repressing transcription through the assembly and modification of chromatin proteins. The NAD(+)-dependent deacetylation of histones by the Sir2p family of proteins lends mechanistic insight into how SIR2 contributes to silencing. Here we describe three locus-specific sir2 mutants that have a spectrum of silencing phenotypes in yeast. These mutants are dependent on SIR1 for silencing function at the HM silent mating-type loci, display distinct phenotypes at the rDNA, and have dominant silencing defects at the telomeres. Telomeric silencing is restored if the mutant proteins are directly tethered to subtelomeric regions, via a Gal4p DNA-binding domain (GBD), or are recruited by tethered GBD-Sir1p. These sir2 mutations are found within conserved residues of the SIR2 family and lead to defects in catalytic activity. Since one of the mutations lies outside the previously defined minimal catalytic core, our results show that additional regions of Sir2p can be important for enzymatic activity and that differences in levels of activity may have distinct effects at the silenced loci.
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