ArticleGenetics2002
A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Biochemical and genetic evidence supports Fyv6 as a second-step splicing factor inRNA (New York, N.Y.) · 2023Article
- Biochemical and Genetic Evidence Supports Fyv6 as a Second-Step Splicing Factor inbioRxiv : the preprint server for biology · 2023Article
- Perturbations of Transcription and Gene Expression-Associated Processes Alter Distribution of Cell Size Values inG3 (Bethesda, Md.) · 2019Article
- Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction.The Journal of biological chemistry · 2016Article
- Review
- Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining.Molecular and cellular biology · 2015Article
- Article
- Yeast DNA ligase IV mutations reveal a nonhomologous end joining function of BRCT1 distinct from XRCC4/Lif1 binding.DNA repair · 2014Article
- Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity.PLoS genetics · 2013Article
- Lif1 SUMOylation and its role in non-homologous end-joining.Nucleic acids research · 2013Article
- Article
- Review
- Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.PLoS genetics · 2010Article
- Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks.PloS one · 2010Article
- Srs2: the "Odd-Job Man" in DNA repair.DNA repair · 2010Review
- MLH1 deficiency enhances tumor cell sensitivity to ganciclovir.Cancer gene therapy · 2009Article
- Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies.The Journal of biological chemistry · 2008Article
- Identification of pathways controlling DNA damage induced mutation in Saccharomyces cerevisiae.DNA repair · 2008Article
- A genome-wide RNAi screen reveals multiple regulators of caspase activation.The Journal of cell biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
We recently described a yeast assay suitable for genetic screening in which simple religation nonhomologous end-joining (NHEJ) and single-strand annealing (SSA) compete for repair of an I-SceI-created double-strand break. Here, the required allele has been introduced into an array of 4781 MATa deletion mutants and each strain screened individually. Two mutants (rad52 and srs2) showed a clear increase in the NHEJ/SSA ratio due to preferential impairment of SSA, but no mutant increased the absolute frequency of NHEJ significantly above the wild-type level. Seven mutants showed a decreased NHEJ/SSA ratio due to frank loss of NHEJ, which corresponded to all known structural/catalytic NHEJ components (yku70, yku80, dnl4, lif1, rad50, mre11, and xrs2); no new mutants in this category were identified. A clearly separable and surprisingly large set of 16 other mutants showed partial defects in NHEJ. Further examination of these revealed that NEJ1 can entirely account for the mating-type regulation of NHEJ, but that this regulatory role was distinct from the postdiauxic/stationary-phase induction of NHEJ that was deficient in other mutants (especially doa1, fyv6, and mck1). These results are discussed in the context of the minimal set of required proteins and regulatory inputs for NHEJ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.