ArticleProceedings of the National Academy of Sciences of the United States of America2002
Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Galpha q/11 pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 164 citations in OpenAlex.
- OPRM1/MRGPRX1 heterodimers drive opioid-induced itch through a peripheral mechanism.Journal of biomedical science · 2026Article
- Evolutionary scenarios for the specific recognition of nonhomologous endogenous peptides by G protein-coupled receptor paralogs.The Journal of biological chemistry · 2025Article
- Article
- Ligand recognition and G protein coupling of the human itch receptor MRGPRX1.Nature communications · 2023Article
- Mechanism of agonist-induced activation of the human itch receptor MRGPRX1.PLoS biology · 2023Article
- Ligand recognition and allosteric modulation of the human MRGPRX1 receptor.Nature chemical biology · 2023Article
- Evaluation of itch and pain induced by bovine adrenal medulla (BAM)8-22, a new human model of non-histaminergic itch.Experimental dermatology · 2022Article
- Lithocholic Acid Activates Mas-Related G Protein-Coupled Receptors, Contributing to Itch in Mice.Biomolecules & therapeutics · 2022Article
- Review
- MRGPRX4 in Cholestatic Pruritus.Seminars in liver disease · 2021Review
- Article
- The signaling pathway and polymorphisms of Mrgprs.Neuroscience letters · 2021Review
- The role of pruriceptors in enhancing sensitivity to pruritogens in a murine chronic compression model of dorsal root ganglion.Molecular brain · 2021Article
- Potential and Realized Impact of Astroglia CaFrontiers in cellular neuroscience · 2021Review
- Advances in Understanding the Initial Steps of Pruritoceptive Itch: How the Itch Hits the Switch.International journal of molecular sciences · 2020Review
- Complementary roles of murine NaScientific reports · 2020Article
- BAM8-22 and its receptor MRGPRX1 may attribute to cholestatic pruritus.Scientific reports · 2019Article
- Protease Activated Receptor-2 Induces Immune Activation and Visceral Hypersensitivity in Post-infectious Irritable Bowel Syndrome Mice.Digestive diseases and sciences · 2019Article
- Effects of intravesical administration of sensory neuron-specific receptor agonist on voiding function in rats with cyclophosphamide-induced cystitis.Central European journal of urology · 2019Article
- Conopeptides promote itch through human itch receptor hMgprX1.Toxicon : official journal of the International Society on Toxinology · 2018Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
MrgA1 and MrgC11 belong to a recently identified family of orphan G-protein coupled receptors, called mrgs (mas-related genes). They are only expressed in a specific subset of sensory neurons that are known to detect painful stimuli. However, the precise physiological function of Mrg receptors and their underlying mechanisms of signal transduction are not known. We therefore have screened a series of neuropeptides against human embryonic kidney (HEK) 293 cells that stably express either MrgA1 or MrgC11 to identify ligands and/or agonists. MrgA1- or MrgC11-specific agonists stimulated dose-dependent increases in intracellular free Ca(2+) in a pertussis toxin-insensitive manner, but failed to alter basal or forskolin-stimulated levels of intracellular cAMP. Furthermore, studies using embryonic fibroblasts derived from various Galpha protein knockout mice demonstrated that both the MrgA1 and MrgC11 receptors are coupled to the Galpha(q/11) signaling pathway. Screening of neuropeptides identified surrogate agonists, most of these peptides included a common C-terminal -RF(Y)G or -RF(Y) amide motif. Structure-function studies suggest that endogenous ligands of Mrg receptors are likely to be RF(Y)G and/or RF(Y) amide-related peptides and that postprocessing of these peptides may serve to determine Mrg receptor-ligand specificity. The differences in ligand specificity also suggest functional diversity amongst the Mrg receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.