ArticleProceedings of the National Academy of Sciences of the United States of America2002
An approach to membrane protein structure without crystals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Gate-controlled proton diffusion and protonation-induced ratchet motion in the stator of the bacterial flagellar motor.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Proton-coupled dynamics in lactose permease.Structure (London, England : 1993) · 2012Article
- Proline-proline-glutamic acid (PPE) protein Rv1168c of Mycobacterium tuberculosis augments transcription from HIV-1 long terminal repeat promoter.The Journal of biological chemistry · 2012Article
- Insights to the evolution of Nucleobase-Ascorbate Transporters (NAT/NCS2 family) from the Cys-scanning analysis of xanthine permease XanQ.International journal of biochemistry and molecular biology · 2012Article
- Lactose permease and the alternating access mechanism.Biochemistry · 2011Article
- An early event in the transport mechanism of LacY protein: interaction between helices V and I.The Journal of biological chemistry · 2011Article
- Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.Biochemistry · 2011Article
- Membrane topology and identification of critical amino acid residues in the Wzx O-antigen translocase from Escherichia coli O157:H4.Journal of bacteriology · 2010Article
- Comparison of human solute carriers.Protein science : a publication of the Protein Society · 2010Article
- Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system.Journal of structural and functional genomics · 2009Article
- GFT projection NMR based resonance assignment of membrane proteins: application to subunit C of E. coli F(1)F (0) ATP synthase in LPPG micelles.Journal of biomolecular NMR · 2008Article
- Site-directed alkylation of LacY: effect of the proton electrochemical gradient.Journal of molecular biology · 2007Article
- Genetic selection for a highly functional cysteine-less membrane protein using site saturation mutagenesis.Analytical biochemistry · 2007Article
- Structural and functional analysis of the Na+/H+ exchanger.The Biochemical journal · 2007Review
- Site-directed alkylation and the alternating access model for LacY.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Sugar binding and protein conformational changes in lactose permease.Biophysical journal · 2006Article
- Amide vibrations are delocalized across the hydrophobic interface of a transmembrane helix dimer.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- Lessons from lactose permease.Annual review of biophysics and biomolecular structure · 2006Review
- Probabilistic cross-link analysis and experiment planning for high-throughput elucidation of protein structure.Protein science : a publication of the Protein Society · 2004Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The lactose permease of Escherichia coli catalyzes coupled translocation of galactosides and H(+) across the cell membrane. It is the best-characterized member of the Major Facilitator Superfamily, a related group of membrane proteins with 12 transmembrane domains that mediate transport of various substrates across cell membranes. Despite decades of effort and their functional importance in all kingdoms of life, no high-resolution structures have been solved for any member of this family. However, extensive biochemical, genetic, and biophysical studies on lactose permease have established its transmembrane topology, secondary structure, and numerous interhelical contacts. Here we demonstrate that this information is sufficient to calculate a structural model at the level of helix packing or better.
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