ArticleProceedings of the National Academy of Sciences of the United States of America2002
Production of transgenic rats by lentiviral transduction of male germ-line stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 82 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed, 242 citations in OpenAlex.
- Non-invasive ovulation tracking enables genetic engineering in wild rodents.Cell reports methods · 2026Article
- Male germ cell technologies in large animals.The Journal of reproduction and development · 2026Review
- Gene therapy for non-obstructive azoospermia: therapeutic avenues and translational pathway to clinical application.Frontiers in genome editing · 2026Review
- Male fertility restoration with stem cell-based therapies: from testicular stem cells to pluripotent stem cell-based in vitro gametogenesis.Fertility and sterility · 2025Review
- Novel Genes of the Male Reproductive System: Potential Roles in Male Reproduction and as Non-hormonal Male Contraceptive Targets.Molecular reproduction and development · 2024Review
- Preservation of fertility in female and male prepubertal patients diagnosed with cancer.Journal of assisted reproduction and genetics · 2023Review
- Review
- Viral Transduction of Mammalian Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Rattus norvegicus Spermatogenesis Colony-Forming Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Generation of surrogate goldfish3 Biotech · 2023Article
- Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis.Cellular and molecular life sciences : CMLS · 2022Article
- Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies†.Biology of reproduction · 2022Review
- Targeted Gene Editing in Porcine Germ Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Spermatogonial Gene Networks Selectively Couple to Glutathione and Pentose Phosphate Metabolism but Not Cysteine Biosynthesis.iScience · 2021Article
- Genetic association and characterization of FSTL5 in isolated clubfoot.Human molecular genetics · 2021Article
- Recent advances: fertility preservation and fertility restoration options for males and females.Faculty reviews · 2021Review
- Targeted Gene Editing in Porcine Spermatogonia.Frontiers in genetics · 2020Article
- Generation of Organized Porcine Testicular Organoids in Solubilized Hydrogels from Decellularized Extracellular Matrix.International journal of molecular sciences · 2019Article
- Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019Article
- Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs.Journal of visualized experiments : JoVE · 2018Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Primary cultures of rat spermatogenic cells that did not bind to collagen matrices were able to colonize and form mature spermatozoa when transferred to testes of recipient males. Up to 73% of the progeny from matings with recipient males were derived from the transferred spermatogenic cells. Subsequently, two populations of germ cells were obtained by selection on laminin matrices. Both populations expressed the spermatogenic cell marker, DAZL, but not the somatic cell marker, vimentin. The cells that bound to laminin represented approximately 5% of the total population and were greatly enriched in ability to colonize a recipient testis, suggesting an enrichment in germ-line stem cells. The colonization potential was maintained for at least 7 days in culture. These cells were subsequently transduced with a lentiviral enhanced GFP reporter vector and then transferred to WT recipient males. After mating, 26 of 44 pups were derived from the cultured donor germ cells, and 13 pups carried the lentiviral transgene. Based on Southern analysis, the transgene was integrated at a different genetic locus in each animal and was transmitted to approximately 50% of pups in the F(2) generation. Thus, by using these procedures, approximately 30% of pups in the F(1) generation inherited and stably transmitted a lentiviral transgene that integrated at various genomic sites.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.