ArticleMolecular and cellular biology2002
Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM.Progress in biophysics and molecular biology · 2021Article
- The Ku complex: recent advances and emerging roles outside of non-homologous end-joining.Cellular and molecular life sciences : CMLS · 2021Review
- Sustained androgen receptor signaling is a determinant of melanoma cell growth potential and tumorigenesis.The Journal of experimental medicine · 2021Article
- The Role of Ku70 as a Cytosolic DNA Sensor in Innate Immunity and Beyond.Frontiers in cellular and infection microbiology · 2021Review
- Proximity Labeling Techniques to Study Chromatin.Frontiers in genetics · 2020Review
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2.Molecular biology of the cell · 2017Article
- Article
- FUS is sequestered in nuclear aggregates in ALS patient fibroblasts.Molecular biology of the cell · 2014Article
- Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.Nucleic acids research · 2014Article
- Emerging roles of the nucleolus in regulating the DNA damage response: the noncanonical DNA repair enzyme APE1/Ref-1 as a paradigmatical example.Antioxidants & redox signaling · 2014Review
- Molecular characterization of DNA repair protein Ku70 from Vitis vinifera and its purification from transgenic tobacco.Transgenic research · 2013Article
- Ku80 attentuates cytotoxicity induced by green fluorescent protein transduction independently of non-homologous end joining.FEBS open bio · 2013Article
- DNA-dependent protein kinase and DNA repair: relevance to Alzheimer's disease.Alzheimer's research & therapy · 2013Review
- Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes.Molecular and cellular biology · 2012Article
- Oxidative DNA damage in neurons: implication of ku in neuronal homeostasis and survival.International journal of cell biology · 2012Article
- Article
- The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.PLoS genetics · 2012Article
- Transcription-induced DNA double strand breaks: both oncogenic force and potential therapeutic target?Clinical cancer research : an official journal of the American Association for Cancer Research · 2011Article
- The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage.Cell death and differentiation · 2011Article
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2 authors.
Funding
Abstract
Ku is an abundant nuclear protein with an essential function in the repair of DNA double-strand breaks. Various observations suggest that Ku also interacts with the cellular transcription machinery, although the mechanism and significance of this interaction are not well understood. In the present study, we investigated the subnuclear distribution of Ku in normally growing human cells by using confocal microscopy, chromatin immunoprecipitation, and protein immunoprecipitation. All three approaches indicated association of Ku with RNA polymerase II (RNAP II) elongation sites. This association occurred independently of the DNA-dependent protein kinase catalytic subunit and was highly selective. There was no detectable association with the initiating isoform of RNAP II or with the general transcription initiation factors. In vitro protein-protein interaction assays demonstrated that the association of Ku with elongation proteins is mediated, in part, by a discrete C-terminal domain in the Ku80 subunit. Functional disruption of this interaction with a dominant-negative mutant inhibited transcription in vitro and in vivo and suppressed cell growth. These results suggest that association of Ku with transcription sites is important for maintenance of global transcription levels. Tethering of double-strand break repair proteins to defined subnuclear structures may also be advantageous in maintenance of genome stability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.