ArticleMolecular and cellular biology2002
Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 78 citations in OpenAlex.
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- A structural perspective of human RNA polymerase III.RNA biology · 2022Article
- 16p12.1 Deletion Orthologs are Expressed in Motile Neural Crest Cells and are Important for Regulating Craniofacial Development inFrontiers in genetics · 2022Article
- Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation.Nucleic acids research · 2021Article
- Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis.PLoS genetics · 2021Article
- Structural insights into transcriptional regulation of human RNA polymerase III.Nature structural & molecular biology · 2021Article
- Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.Nature structural & molecular biology · 2021Article
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- Robust repression of tRNA gene transcription during stress requires protein arginine methylation.Life science alliance · 2019Article
- The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation.Nucleic acids research · 2018Article
- Transcriptional interference by RNA polymerase III affects expression of theGenes & development · 2017Article
- Transcribing RNA polymerase III observed by electron cryomicroscopy.The FEBS journal · 2016Review
- Virus-induced gene silencing of the RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana.Scientific reports · 2016Article
- Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit.Nucleic acids research · 2015Article
- Review
- Screen for mitochondrial DNA copy number maintenance genes reveals essential role for ATP synthase.Molecular systems biology · 2014Article
- Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.Transcription · 2014Review
- Gene duplication and neofunctionalization: POLR3G and POLR3GL.Genome research · 2014Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of similarity to the yeast subunits. We have purified an RNA polymerase III complex and identified its components. We found that two RNA polymerase III subunits, referred to as RPC8 and RPC9, displayed sequence similarity to the RNA polymerase II RPB7 and RPB4 subunits, respectively. RPC8 and RPC9 associated with each other, paralleling the association of the RNA polymerase II subunits, and were thus paralogues of RPB7 and RPB4. Furthermore, the complex contained a prominent 80-kDa polypeptide, which we called RPC5 and which corresponded to the human orthologue of the yeast C37 subunit despite limited sequence similarity. RPC5 associated with RPC53, the human orthologue of S. cerevisiae C53, paralleling the association of the S. cerevisiae C37 and C53 subunits, and was required for transcription from the type 2 VAI and type 3 human U6 promoters. Our results provide a characterization of human RNA polymerase III and show that the RPC5 subunit is essential for transcription.
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