ArticleMolecular and cellular biology2002
Native and recombinant polycomb group complexes establish a selective block to template accessibility to repress transcription in vitro.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 129 citations in OpenAlex.
- More than SAM: mechanisms of action of the Polycomb group protein polyhomeotic.Nucleus (Austin, Tex.) · 2026Review
- H2AK119ub dynamics control hair follicle stem cell quiescence.Nature communications · 2025Article
- A Two-Step Mechanism for Creating Stable, Condensed Chromatin with the Polycomb Complex PRC1.Molecules (Basel, Switzerland) · 2024Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Plant BBR/BPC transcription factors: unlocking multilayered regulation in development, stress and immunity.Planta · 2023Review
- Polycomb group genes are required for neuronal pruning in Drosophila.BMC biology · 2023Article
- PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency.Nature structural & molecular biology · 2021Article
- The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability.International journal of molecular sciences · 2021Review
- Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy.Nature communications · 2021Article
- Article
- Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis.Nucleic acids research · 2018Article
- An Unexpected Regulatory Cascade Governs a Core Function of the Drosophila PRC1 Chromatin Protein Su(z)2.Genetics · 2017Article
- dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.PLoS genetics · 2016Article
- Kicking against the PRCs - A Domesticated Transposase Antagonises Silencing Mediated by Polycomb Group Proteins and Is an Accessory Component of Polycomb Repressive Complex 2.PLoS genetics · 2015Article
- Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.Genes & development · 2015Article
- Transcriptional silencing by polycomb-group proteins.Cold Spring Harbor perspectives in biology · 2014Review
- Heterochromatic gene silencing by activator interference and a transcription elongation barrier.The Journal of biological chemistry · 2013Article
- Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.Molecular cell · 2013Review
- Polycomb repressive complex 1 (PRC1) disassembles RNA polymerase II preinitiation complexes.The Journal of biological chemistry · 2012Article
- A bridging model for persistence of a polycomb group protein complex through DNA replication in vitro.Molecular cell · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polycomb group (PcG) proteins are responsible for stable repression of homeotic gene expression during Drosophila melanogaster development. They are thought to stabilize chromatin structure to prevent transcription, though how they do this is unknown. We have established an in vitro system in which the PcG complex PRC1 and a recombinant PRC1 core complex (PCC) containing only PcG proteins are able to repress transcription by both RNA polymerase II and by T7 RNA polymerase. We find that assembly of the template into nucleosomes enhances repression by PRC1 and PCC. The subunit Psc is able to inhibit transcription on its own. PRC1- and PCC-repressed templates remain accessible to Gal4-VP16 binding, and incubation of the template with HeLa nuclear extract before the addition of PCC eliminates PCC repression. These results suggest that PcG proteins do not merely prohibit all transcription machinery from binding the template but instead likely inhibit specific steps in the transcription reaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.