ArticleClinical and experimental immunology2002
Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
Article in Clinical and experimental immunology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 98 citations in OpenAlex.
- The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP.Angiogenesis · 2024Article
- Effects of continuous positive airway pressure on comprehensive geriatric assessment and cognitive function in elderly patients with obstructive sleep apnea syndrome.Internal and emergency medicine · 2023Article
- The Variable Nature of Vitamin C-Does It Help When Dealing with Coronavirus?Antioxidants (Basel, Switzerland) · 2022Review
- LOX-1 is a poor prognostic indicator and induces epithelial-mesenchymal transition and metastasis in pancreatic cancer patients.Cellular oncology (Dordrecht, Netherlands) · 2018Article
- Inflammatory response mechanisms exacerbating hypoxemia in coexistent pulmonary fibrosis and sleep apnea.Mediators of inflammation · 2015Review
- Neurotoxic saboteurs: straws that break the hippo's (hippocampus) back drive cognitive impairment and Alzheimer's Disease.Neurotoxicity research · 2013Review
- Quintessential risk factors: their role in promoting cognitive dysfunction and Alzheimer's disease.Neurochemical research · 2012Review
- Etiopathogenetic mechanisms of pulmonary hypertension in sleep-related breathing disorders.Pulmonary medicine · 2012Article
- Pathogenesis of cognitive dysfunction in patients with obstructive sleep apnea: a hypothesis with emphasis on the nucleus tractus solitarius.Sleep disorders · 2012Article
- A proteomic analysis of C-reactive protein stimulated THP-1 monocytes.Proteome science · 2011Article
- Protein expression profiles distinguish between experimental invasive pulmonary aspergillosis and Pseudomonas pneumonia.Proteomics · 2010Article
- Suppression of Plasmodium cynomolgi in rhesus macaques by coinfection with Babesia microti.Infection and immunity · 2010Article
- The evolving role of C-reactive protein in atherothrombosis.Clinical chemistry · 2009Review
- New evidences for C-reactive protein (CRP) deposits in the arterial intima as a cardiovascular risk factor.Clinical interventions in aging · 2008Review
- Advanced glycation of apolipoprotein A-I impairs its anti-atherogenic properties.Diabetologia · 2007Article
- Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction.Circulation research · 2006Article
- Role of C-reactive protein in complement-mediated hemolysis in Malaria.Glycoconjugate journal · 2006Article
- Role of leptin in atherogenesis.Experimental and clinical cardiology · 2006Article
- Expression of genes encoding innate host defense molecules in normal human monocytes in response to Candida albicans.Infection and immunity · 2005Article
- Evidence for C-reactive protein's role in (CRP) vascular disease: atherothrombosis, immuno-regulation and CRP.Journal of thrombosis and thrombolysis · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C-reactive protein (CRP) is the prototypic acute phase serum protein in humans. The effects of CRP on primary human monocyte adhesion molecule expression and interaction with the endothelium have not been studied. Herein, we describe an investigation into the phenotypic and functional consequences of CRP binding to peripheral blood monocytes ex vivo. Peripheral whole blood was collected from healthy, non-smoking males. Mononuclear cells (MNC) and monocytes were isolated by differential centrifugation using lymphoprep and Dynal negative isolation kit, respectively. Cells were exposed to CRP from 0 to 250 micro g/ml for 0-60 min at 37 degrees C and analysed for (a) CD11b, PECAM-1 (CD31) and CD32 expression by flow cytometry and (b) adhesion to LPS (1 micro g/ml; 0-24 h) treated human umbilical vein endothelial cells (HUVEC). CD14+ monocyte expression of CD11b increased significantly up to twofold when exposed to CRP, compared to controls. There was no significant difference in CD32 expression, whereas CD31 expression decreased after exposure to CRP. CRP treatment of monocytes inhibited their adhesion to early LPS-activated HUVEC (0-5 h). However, the adhesion of CRP-treated monocytes to HUVEC was significantly greater to late activation antigens on HUVEC (24 h, LPS) compared to controls. We have shown that CRP can affect monocyte activation ex vivo and induce phenotypic changes that result in an altered recruitment to endothelial cells. This study provides the first evidence for a further role for C-reactive protein in both monocyte activation and adhesion, which may be of importance during an inflammatory event.
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