ArticleMolecular biology of the cell2002
Mitochondrial development during life cycle differentiation of African trypanosomes: evidence for a kinetoplast-dependent differentiation control point.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes.Nucleic acids research · 2026Article
- Article
- Redesigned and reversed: architectural and functional oddities of the trypanosomal ATP synthase.Parasitology · 2021Review
- Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome.PloS one · 2021Article
- An Alba-domain protein required for proteome remodelling during trypanosome differentiation and host transition.PLoS pathogens · 2021Article
- Mitochondrial DNA is critical for longevity and metabolism of transmission stage Trypanosoma brucei.PLoS pathogens · 2018Article
- Inositol polyphosphate multikinase regulation of Trypanosoma brucei life stage development.Molecular biology of the cell · 2018Article
- Molecular Characterization ofFrontiers in cellular and infection microbiology · 2018Article
- The AMPKα1 Pathway Positively Regulates the Developmental Transition from Proliferation to Quiescence in Trypanosoma brucei.Cell reports · 2016Article
- The krebs cycle enzyme α-ketoglutarate decarboxylase is an essential glycosomal protein in bloodstream African trypanosomes.Eukaryotic cell · 2015Article
- Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei.PloS one · 2015Article
- Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Single point mutations in ATP synthase compensate for mitochondrial genome loss in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Three mitochondrial DNA polymerases are essential for kinetoplast DNA replication and survival of bloodstream form Trypanosoma brucei.Eukaryotic cell · 2011Article
- Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei.Molecular microbiology · 2008Article
- RNA editing in Trypanosoma brucei requires three different editosomes.Molecular and cellular biology · 2008Article
- Hydrolysis products of cAMP analogs cause transformation of Trypanosoma brucei from slender to stumpy-like forms.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Chromosome-wide analysis of gene function by RNA interference in the african trypanosome.Eukaryotic cell · 2006Article
- The F1-ATP synthase complex in bloodstream stage trypanosomes has an unusual and essential function.The EMBO journal · 2005Article
- Article
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4 authors.
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Abstract
Life cycle differentiation of African trypanosomes entails developmental regulation of mitochondrial activity. This requires regulation of the nuclear genome and the kinetoplast, the trypanosome's unusual mitochondrial genome. To investigate the potential cross talk between the nuclear and mitochondrial genome during the events of differentiation, we have 1) disrupted expression of a nuclear-encoded component of the cytochrome oxidase (COX) complex; and 2) generated dyskinetoplastid cells, which lack a mitochondrial genome. Using RNA interference (RNAi) and by disrupting the nuclear COX VI gene, we demonstrate independent regulation of COX component mRNAs encoded in the nucleus and kinetoplast. However, two independent approaches (acriflavine treatment and RNA interference ablation of mitochondrial topoisomerase II) failed to establish clonal lines of dyskinetoplastid bloodstream forms. Nevertheless, dyskinetoplastid forms generated in vivo could undergo two life cycle differentiation events: transition from bloodstream slender to stumpy forms and the initiation of transformation to procyclic forms. However, they subsequently arrested at a specific point in this developmental program before cell cycle reentry. These results provide strong evidence for a requirement for kinetoplast DNA in the bloodstream and for a kinetoplast-dependent control point during differentiation to procyclic forms.
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