ArticleMolecular biology of the cell2002
Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 99 citations in OpenAlex.
- Chromosomal Roadblocks in Male Fertility: Mechanisms, Risk Factors and Syndromes.Medicina (Kaunas, Lithuania) · 2025Review
- Checkpoint Proteins Bub1 and Bub3 Delay Anaphase Onset in Response to Low Tension Independent of Microtubule-Kinetochore Detachment.Cell reports · 2019Article
- Protein arginine methylation: an emerging regulator of the cell cycle.Cell division · 2018Review
- Oxidative Stress Delays Prometaphase/Metaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint.Oxidative medicine and cellular longevity · 2017Article
- Antimitotic drugs in the treatment of cancer.Cancer chemotherapy and pharmacology · 2015Review
- Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint.Nature communications · 2015Article
- Stressing mitosis to death.Frontiers in oncology · 2014Review
- NEK2 induces drug resistance mainly through activation of efflux drug pumps and is associated with poor prognosis in myeloma and other cancers.Cancer cell · 2013Article
- Bub1 and BubR1: at the interface between chromosome attachment and the spindle checkpoint.Molecular and cellular biology · 2011Review
- Caenorhabditis elegans cyclin B3 is required for multiple mitotic processes including alleviation of a spindle checkpoint-dependent block in anaphase chromosome segregation.PLoS genetics · 2010Article
- Re-evaluating the role of Tao1 in the spindle checkpoint.Chromosoma · 2010Article
- Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster.Molecular and cellular biology · 2010Article
- Mitotic chromosomal instability and cancer: mouse modelling of the human disease.Nature reviews. Cancer · 2010Review
- Smurf2 as a novel mitotic regulator: From the spindle assembly checkpoint to tumorigenesis.Cell division · 2009Article
- Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint.The EMBO journal · 2009Article
- Review
- Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis.The Journal of cell biology · 2008Article
- Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit.The Journal of cell biology · 2008Article
- The chromosomal passenger complex and the spindle assembly checkpoint: kinetochore-microtubule error correction and beyond.Cell division · 2008Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The spindle checkpoint monitors microtubule attachment and tension at kinetochores to ensure proper chromosome segregation. Previously, PtK1 cells in hypothermic conditions (23 degrees C) were shown to have a pronounced mitotic delay, despite having normal numbers of kinetochore microtubules. At 23 degrees C, we found that PtK1 cells remained in metaphase for an average of 101 min, compared with 21 min for cells at 37 degrees C. The metaphase delay at 23 degrees C was abrogated by injection of Mad2 inhibitors, showing that Mad2 and the spindle checkpoint were responsible for the prolonged metaphase. Live cell imaging showed that kinetochore Mad2 became undetectable soon after chromosome congression. Measurements of the stretch between sister kinetochores at metaphase found a 24% decrease in tension at 23 degrees C, and metaphase kinetochores at 23 degrees C exhibited higher levels of 3F3/2, Bub1, and BubR1 compared with 37 degrees C. Microinjection of anti-BubR1 antibody abolished the metaphase delay at 23 degrees C, indicating that the higher kinetochore levels of BubR1 may contribute to the delay. Disrupting both Mad2 and BubR1 function induced anaphase with the same timing as single inhibitions, suggesting that these checkpoint genes function in the same pathway. We conclude that reduced tension at kinetochores with a full complement of kinetochore microtubules induces a checkpoint dependent metaphase delay associated with elevated amounts of kinetochore 3F3/2, Bub1, and BubR1 labeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.