ArticleMolecular biology of the cell2002
ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Sorting of lysosomal enzyme and autophagy are regulated by the GGA1-induced TGN lipid scrambling.Science advances · 2026Article
- Arfs on the Golgi: four conductors, one orchestra.Frontiers in molecular biosciences · 2025Review
- ADP-Ribosylation Factor Family of Small GTP-Binding Proteins: Their Membrane Recruitment, Activation, Crosstalk and Functions.Frontiers in cell and developmental biology · 2022Review
- FAM134B oligomerization drives endoplasmic reticulum membrane scission for ER-phagy.The EMBO journal · 2020Article
- Adaptor protein complex-1 (AP-1) is recruited by the HEATR5 protein Laa1 and its co-factor Laa2 in yeast.The Journal of biological chemistry · 2019Article
- Visualization of Protein Sorting at theFrontiers in cell and developmental biology · 2019Article
- Alternative Splicing in Ca(V)2.2 Regulates Neuronal Trafficking via Adaptor Protein Complex-1 Adaptor Protein Motifs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Glucose starvation inhibits autophagy via vacuolar hydrolysis and induces plasma membrane internalization by down-regulating recycling.The Journal of biological chemistry · 2014Article
- Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1.Cell · 2013Article
- Article
- A novel role for retromer in the control of epithelial cell polarity.Communicative & integrative biology · 2011Article
- AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles.Molecular biology of the cell · 2009Article
- Clathrin regulates the association of PIPKIgamma661 with the AP-2 adaptor beta2 appendage.The Journal of biological chemistry · 2009Article
- Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.Archives of biochemistry and biophysics · 2008Article
- Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.The Journal of cell biology · 2008Article
- Human immunodeficiency virus type 1 Nef: adapting to intracellular trafficking pathways.Microbiology and molecular biology reviews : MMBR · 2006Review
- Proteomic analysis of adaptor protein 1A coats selectively assembled on liposomes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis.Molecular biology of the cell · 2005Article
- In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5.Molecular biology of the cell · 2004Article
- Crystal structure of the clathrin adaptor protein 1 core.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At the trans-Golgi network, clathrin coats containing AP-1 adaptor complexes are formed in an ARF1-dependent manner, generating vesicles transporting cargo proteins to endosomes. The mechanism of site-specific targeting of AP-1 and the role of cargo are poorly understood. We have developed an in vitro assay to study the recruitment of purified AP-1 adaptors to chemically defined liposomes presenting peptides corresponding to tyrosine-based sorting motifs. AP-1 recruitment was found to be dependent on myristoylated ARF1, GTP or nonhydrolyzable GTP-analogs, tyrosine signals, and small amounts of phosphoinositides, most prominently phosphatidylinositol 4,5-bisphosphate, in the absence of any additional cytosolic or membrane bound proteins. AP-1 from cytosol could be recruited to a tyrosine signal independently of the lipid composition, but the rate of recruitment was increased by phosphatidylinositol 4,5-bisphosphate. The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.