ArticleMolecular biology of the cell2002
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 204 citations in OpenAlex.
- Article
- The GTP-tubulin cap is not the determinant of microtubule end stability in cells.Molecular biology of the cell · 2024Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons.Journal of visualized experiments : JoVE · 2021Article
- Novel function ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Microtubule-dependent pushing forces contribute to long-distance aster movement and centration inMolecular biology of the cell · 2020Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Search and Capture Efficiency of Dynamic Microtubules for Centrosome Relocation during IS Formation.Biophysical journal · 2019Article
- GTP-binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition.Scientific reports · 2018Article
- Update: Plant Cortical Microtubule Arrays.Plant physiology · 2018Review
- The DNA replication protein Cdc6 inhibits the microtubule-organizing activity of the centrosome.The Journal of biological chemistry · 2017Article
- HeLife · 2017Article
- Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2.The Journal of biological chemistry · 2017Article
- Article
- Gene Network Construction from Microarray Data Identifies a Key Network Module and Several Candidate Hub Genes in Age-Associated Spatial Learning Impairment.Frontiers in systems neuroscience · 2017Article
- Heterogeneous architecture of vertebrate kinetochores revealed by three-dimensional superresolution fluorescence microscopy.Molecular biology of the cell · 2016Article
- EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice.Nature communications · 2016Article
- Identification of microtubule growth deceleration and its regulation by conserved and novel proteins.Molecular biology of the cell · 2016Article
- Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability.Nature communications · 2016Article
- Negative regulation of EB1 turnover at microtubule plus ends by interaction with microtubule-associated protein ATIP3.Oncotarget · 2015Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
EB1 targets to polymerizing microtubule ends, where it is favorably positioned to regulate microtubule polymerization and confer molecular recognition of the microtubule end. In this study, we focus on two aspects of the EB1-microtubule interaction: regulation of microtubule dynamics by EB1 and the mechanism of EB1 association with microtubules. Immunodepletion of EB1 from cytostatic factor-arrested M-phase Xenopus egg extracts dramatically reduced microtubule length; this was complemented by readdition of EB1. By time-lapse microscopy, EB1 increased the frequency of microtubule rescues and decreased catastrophes, resulting in increased polymerization and decreased depolymerization and pausing. Imaging of EB1 fluorescence revealed a novel structure: filamentous extensions on microtubule plus ends that appeared during microtubule pauses; loss of these extensions correlated with the abrupt onset of polymerization. Fluorescent EB1 localized to comets at the polymerizing plus ends of microtubules in cytostatic factor extracts and uniformly along the lengths of microtubules in interphase extracts. The temporal decay of EB1 fluorescence from polymerizing microtubule plus ends predicted a dissociation half-life of seconds. Fluorescence recovery after photobleaching also revealed dissociation and rebinding of EB1 to the microtubule wall with a similar half-life. EB1 targeting to microtubules is thus described by a combination of higher affinity binding to polymerizing ends and lower affinity binding along the wall, with continuous dissociation. The latter is likely to be attenuated in interphase. The highly conserved effect of EB1 on microtubule dynamics suggests it belongs to a core set of regulatory factors conserved in higher organisms, and the complex pattern of EB1 targeting to microtubules could be exploited by the cell for coordinating microtubule behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.