ArticleMolecular biology of the cell2002
High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 139 citations in OpenAlex.
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- Real-time visualization reveals Mycobacterium tuberculosis ESAT-6 disrupts phagosome-like compartment via fibril-mediated vesiculation.Cell reports · 2025Article
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- Gene expression dynamics of natural assemblages of heterotrophic flagellates during bacterivory.Microbiome · 2023Article
- Diesel exhaust exposure alters the expression of networks implicated in neurodegeneration in zebrafish brains.Cell biology and toxicology · 2023Article
- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comparative Transcriptome Analysis of Head Kidney of Aeromonas hydrophila-infected Hypoxia-tolerant and Normal Large Yellow Croaker.Marine biotechnology (New York, N.Y.) · 2022Article
- Kinetics of phagosome maturation is coupled to their intracellular motility.Communications biology · 2022Article
- Review
- Phagosome resolution regenerates lysosomes and maintains the degradative capacity in phagocytes.The Journal of cell biology · 2021Article
- Article
- Dinoflagellates alter their carbon and nutrient metabolic strategies across environmental gradients in the central Pacific Ocean.Nature microbiology · 2021Article
- Gene expression during bacterivorous growth of a widespread marine heterotrophic flagellate.The ISME journal · 2021Article
- Coordinated Ras and Rac Activity Shapes Macropinocytic Cups and Enables Phagocytosis of Geometrically Diverse Bacteria.Current biology : CB · 2020Article
- From Species to Regional and Local Specialization of Intestinal Macrophages.Frontiers in cell and developmental biology · 2020Review
- Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts by Legionella pneumophila.Cellular microbiology · 2019Review
- Eat Prey, Live:Frontiers in immunology · 2017Review
- Isolation ofFrontiers in cellular and infection microbiology · 2017Article
- Evolution of Cell-Autonomous Effector Mechanisms in Macrophages versus Non-Immune Cells.Microbiology spectrum · 2016Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular mechanisms of endocytosis in the genetically and biochemically tractable professional phagocyte Dictyostelium discoideum reveal a striking degree of similarity to higher eukaryotic cells. Pulse-chase feeding with latex beads allowed purification of phagosomes at different stages of maturation. Gentle ATP stripping of an actin meshwork entrapping contaminating organelles resulted in a 10-fold increase in yield and purity, as confirmed by electron microscopy. Temporal profiling of signaling, cytoskeletal, and trafficking proteins resulted in a complex molecular fingerprint of phagosome biogenesis and maturation. First, nascent phagosomes were associated with coronin and rapidly received a lysosomal glycoprotein, LmpB. Second, at least two phases of delivery of lysosomal hydrolases (cathepsin D [CatD] and cysteine protease [CPp34]) were accompanied by removal of plasma membrane components (PM4C4 and biotinylated surface proteins). Third, a phase of late maturation, preparing for final exocytosis of undigested material, included quantitative recycling of hydrolases and association with vacuolin. Also, lysosomal glycoproteins of the Lmp family showed distinct trafficking kinetics. The delivery and recycling of CatD was directly visualized by confocal microscopy. This heavy membrane traffic of cargos was precisely accompanied by regulatory proteins such as the Rab7 GTPases and the endosomal SNAREs Vti1 and VAMP7. This initial molecular description of phagocytosis demonstrates the feasibility of a comprehensive analysis of phagosomal lipids and proteins in genetically modified strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.