ArticleMolecular biology of the cell2002
Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 77 citations in OpenAlex.
- Mitotic error correction and the spindle assembly checkpoint: a tension-filled relationship.Cell cycle (Georgetown, Tex.) · 2026Review
- Suppression of chromosome instability by targeting a DNA helicase in budding yeast.Molecular biology of the cell · 2023Article
- PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis.Cellular and molecular life sciences : CMLS · 2022Article
- SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.The Journal of cell biology · 2021Article
- A CRISPR/Cas9-mediated in situ complementation method for Phytophthora sojae mutants.Molecular plant pathology · 2021Article
- PP2AJournal of cell science · 2020Article
- PP2A Functions during Mitosis and Cytokinesis in Yeasts.International journal of molecular sciences · 2019Review
- Connecting GCN5's centromeric SAGA to the mitotic tension-sensing checkpoint.Molecular biology of the cell · 2018Article
- Protein Kinase C Controls Binding of Igo/ENSA Proteins to Protein Phosphatase 2A in Budding Yeast.The Journal of biological chemistry · 2017Article
- Article
- All roads lead to PP2A: exploiting the therapeutic potential of this phosphatase.The FEBS journal · 2016Review
- Article
- Zds1/Zds2-PP2ACdc55 complex specifies signaling output from Rho1 GTPase.The Journal of cell biology · 2016Article
- Protein phosphatase 2A is involved in the tyrosine hydroxylase phosphorylation regulated by α-synuclein.Neurochemical research · 2015Article
- Sgo1 recruits PP2A to chromosomes to ensure sister chromatid bi-orientation during mitosis.Journal of cell science · 2014Article
- Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation.PLoS genetics · 2014Article
- Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast.Cell cycle (Georgetown, Tex.) · 2014Article
- Visualization of subunit interactions and ternary complexes of protein phosphatase 2A in mammalian cells.PloS one · 2014Article
- An integrated overview of spatiotemporal organization and regulation in mitosis in terms of the proteins in the functional supercomplexes.Frontiers in microbiology · 2014Review
- Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein phosphatase 2A (PP2A) regulates a broad spectrum of cellular processes. This enzyme is a collection of varied heterotrimeric complexes, each composed of a catalytic (C) and regulatory (B) subunit bound together by a structural (A) subunit. To understand the cell cycle dynamics of this enzyme population, we carried out quantitative and qualitative analyses of the PP2A subunits of Saccharomyces cerevisiae. We found the following: the level of each subunit remained constant throughout the cell cycle; there is at least 10 times more of one of the regulatory subunits (Rts1p) than the other (Cdc55p); Tpd3p, the structural subunit, is limiting for both catalytic and regulatory subunit binding. Using green fluorescent protein-tagged forms of each subunit, we monitored the sites of significant accumulation of each protein throughout the cell cycle. The two regulatory subunits displayed distinctly different dynamic localization patterns that overlap with the A and C subunits at the bud tip, kinetochore, bud neck, and nucleus. Using strains null for single subunit genes, we confirmed the hypothesis that regulatory subunits determine sites of PP2A accumulation. Although Rts1p and Tpd3p required heterotrimer formation to achieve normal localization, Cdc55p achieved its normal localization in the absence of either an A or C subunit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.