ArticleJournal of virology2002
Role of the alfalfa mosaic virus methyltransferase-like domain in negative-strand RNA synthesis.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Inhibition of Chikungunya virus by an adenosine analog targeting the SAM-dependent nsP1 methyltransferase.FEBS letters · 2020Article
- Mutational analysis of hepatitis E virus ORF1 "Y-domain": Effects on RNA replication and virion infectivity.World journal of gastroenterology · 2017Article
- Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing.PloS one · 2017Article
- Sequence analysis reveals a conserved extension in the capping enzyme of the alphavirus supergroup, and a homologous domain in nodaviruses.Biology direct · 2015Article
- Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato.Virus genes · 2009Article
- cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication.Journal of virology · 2008Article
- Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3.Structure (London, England : 1993) · 2005Article
- Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.Journal of virology · 2005Article
- Similarities and differences between the subgenomic and minus-strand promoters of an RNA plant virus.Journal of virology · 2004Article
- A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis.Journal of virology · 2003Article
- Coordinate replication of alfalfa mosaic virus RNAs 1 and 2 involves cis- and trans-acting functions of the encoded helicase-like and polymerase-like domains.Journal of virology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNAs 1 and 2 of the tripartite genome of alfalfa mosaic virus (AMV) encode the replicase proteins P1 and P2, respectively. P1 contains a methyltransferase-like domain in its N-terminal half, which has a putative role in capping the viral RNAs. Six residues in this domain that are highly conserved in the methyltransferase domains of alphavirus-like viruses were mutated individually in AMV P1. None of the mutants was infectious to plants. Mutant RNA 1 was coexpressed with wild-type (wt) RNAs 2 and 3 from transferred DNA vectors in Nicotiana benthamiana by agroinfiltration. Mutation of His-100 or Cys-189 in P1 reduced accumulation of negative- and positive-strand RNA in the infiltrated leaves to virtually undetectable levels. Mutation of Asp-154, Arg-157, Cys-182, or Tyr-266 in P1 reduced negative-strand RNA accumulation to levels ranging from 2 to 38% of those for the wt control, whereas positive-strand RNA accumulation by these mutants was 2% or less. The (transiently) expressed replicases of the six mutants were purified from the agroinfiltrated leaves. Polymerase activities of these preparations in vitro ranged from undetectable to wt levels. The data indicate that, in addition to its putative role in RNA capping, the methyltransferase-like domain of P1 has distinct roles in replication-associated functions required for negative-strand RNA synthesis. The defect in negative-strand RNA synthesis of the His-100 and Cys-189 mutants could be complemented in trans by coexpression of wt P1.
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