ArticleJournal of virology2002
Porcine endogenous retrovirus infects but does not replicate in nonhuman primate primary cells and cell lines.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 68 citations in OpenAlex.
- Surveillance and prevention of infection in clinical xenotransplantation.Clinical microbiology reviews · 2025Review
- Corneal xenotransplantation: Where are we standing?Progress in retinal and eye research · 2021Review
- Infection in xenotransplantation: opportunities and challenges.Current opinion in organ transplantation · 2019Review
- Why was PERV not transmitted during preclinical and clinical xenotransplantation trials and after inoculation of animals?Retrovirology · 2018Review
- Long-term safety from transmission of porcine endogenous retrovirus after pig-to-non-human primate corneal transplantation.Xenotransplantation · 2017Article
- No expression of porcine endogenous retrovirus after pig to monkey xenotransplantation.Laboratory animal research · 2014Article
- Infection barriers to successful xenotransplantation focusing on porcine endogenous retroviruses.Clinical microbiology reviews · 2012Review
- Suboptimal porcine endogenous retrovirus infection in non-human primate cells: implication for preclinical xenotransplantation.PloS one · 2010Article
- Fluidized-bed bioartificial liver assist devices (BLADs) based on microencapsulated primary porcine hepatocytes have risk of porcine endogenous retroviruses transmission.Hepatology international · 2010Article
- Retroviral restriction factors and infectious risk in xenotransplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2010Article
- Absence of replication of porcine endogenous retrovirus and porcine lymphotropic herpesvirus type 1 with prolonged pig cell microchimerism after pig-to-baboon xenotransplantation.Journal of virology · 2008Article
- Porcine endogenous retrovirus integration sites in the human genome: features in common with those of murine leukemia virus.Journal of virology · 2006Article
- Mice transgenic for a human porcine endogenous retrovirus receptor are susceptible to productive viral infection.Journal of virology · 2006Article
- Determinants of high titer in recombinant porcine endogenous retroviruses.Journal of virology · 2004Article
- Xenotransplantation: infectious risk revisited.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2004Review
- Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice.The Journal of clinical investigation · 2004Article
- Identification of receptors for pig endogenous retrovirus.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Porcine endogenous retroviruses (PERV) can infect human cell lines in vitro; hence, there is a presumed risk of viral exposure to a recipient when pig cells are transplanted into humans (xenotransplantation). Nonhuman primates (NHP) are considered a potential permissive animal model to study the risk of in vivo infection of PERV after xenotransplantation. We set out to determine whether PERV can infect and replicate in NHP primary cells or established cell lines from African green monkey, rhesus macaque, and baboon. We confirm that the NHP cell lines under investigation were infected with PERV as measured by detection of viral DNA and RNA by PCR and reverse transcription (RT)-PCR, respectively, indicating that a functional receptor must be present on the cell surface. However, the load of detectable viral DNA in infected NHP cells declined over time, and the cells never had detectable reverse transcriptase activity. Utilizing quantitative real-time TaqMan PCR we found detectable levels of unintegrated DNA intermediates, but the levels were approximately 100-fold lower compared to HEK 293 cells infected with PERV. Virions released from infected NHP cells could productively infect naïve human cell lines, HEK 293 and HeLa, as shown by RT-PCR and RT assay. However, naïve NHP cells remained negative in RT-PCR and RT assay after exposure to virions from infected NHP cells. Together our data demonstrate that NHP cells are not permissive to productive replication by PERV, presumably due to inefficient cell entry and replication. In light of these observations, the appropriateness of NHP as suitable animal models to study PERV infection in vivo needs to be reevaluated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.