ArticleProceedings of the National Academy of Sciences of the United States of America2002
Glucose is toxic to glycosome-deficient trypanosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 126 citations in OpenAlex.
- Ultrastructural expansion microscopy reveals unexpected levels of glycosome heterogeneity in African trypanosomes.Journal of microscopy · 2026Article
- Cross-species comparisons in a unified medium suggest broadly stable glycosome-linked enzyme levels under nutrient and oxygen variation.Frontiers in parasitology · 2026Article
- Endosymbiosis in trypanosomatids: the bacterium regulates the intermediate and oxidative metabolism of the host cell.mSphere · 2025Article
- Glycolysis model shows that allostery maintains high ATP and limits accumulation of intermediates.Biophysical journal · 2025Article
- PEX1 is essential for glycosome biogenesis and trypanosomatid parasite survival.Frontiers in cellular and infection microbiology · 2024Article
- Small molecule mediated inhibition of protein cargo recognition by peroxisomal transport receptor PEX5 is toxic to Trypanosoma.Scientific reports · 2022Article
- Novel Trypanocidal Inhibitors that Block Glycosome Biogenesis by Targeting PEX3-PEX19 Interaction.Frontiers in cell and developmental biology · 2021Article
- Article
- Cellular Markers for the Identification of Chemoresistant Isolates in Leishmania.Methods in molecular biology (Clifton, N.J.) · 2020Article
- The early-acting glycosome biogenic protein Pex3 is essential for trypanosome viability.Life science alliance · 2019Article
- Inositol polyphosphate multikinase regulation of Trypanosoma brucei life stage development.Molecular biology of the cell · 2018Article
- Spatial Organization of Metabolic Enzyme Complexes in Cells.Biochemistry · 2017Review
- Glycosome biogenesis in trypanosomes and the de novo dilemma.PLoS neglected tropical diseases · 2017Review
- Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum.The Journal of eukaryotic microbiology · 2017Article
- An HTRF based high-throughput screening for discovering chemical compounds that inhibit the interaction betweenBiochemistry and biophysics reports · 2016Article
- Comprehensive proteomics analysis of glycosomes from Leishmania donovani.Omics : a journal of integrative biology · 2015Article
- ATG24 Represses Autophagy and Differentiation and Is Essential for Homeostasy of the Flagellar Pocket in Trypanosoma brucei.PloS one · 2015Article
- Using fluorescent proteins to monitor glycosome dynamics in the African trypanosome.Journal of visualized experiments : JoVE · 2014Article
- Contribution of pyruvate phosphate dikinase in the maintenance of the glycosomal ATP/ADP balance in the Trypanosoma brucei procyclic form.The Journal of biological chemistry · 2014Article
- Evidence for loss of a partial flagellar glycolytic pathway during trypanosomatid evolution.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Trypanosomatids, the etiologic agents of sleeping sickness, leishmaniasis, and Chagas' disease, compartmentalize glycolysis within glycosomes, metabolic organelles related to peroxisomes. Here, we identify a trypanosome homologue of PEX14, one of the components of the peroxisomal protein import docking complex. We have used double-stranded RNA interference to target the PEX14 transcript for degradation. Glycosomal matrix protein import was compromised, and both glycolytic bloodstream stage parasites and mitochondrially respiring procyclic stage parasites were killed. Thus, unlike peroxisomes, glycosomes are essential organelles. Surprisingly, procyclic forms, which can grow in the absence of glucose, were killed by PEX14 RNA interference only when simple sugars were present. Thus, interference with glycosome protein import makes glucose toxic to trypanosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.