ArticleProceedings of the National Academy of Sciences of the United States of America2002
Genome fragment of Wolbachia endosymbiont transferred to X chromosome of host insect.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.
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128 citing papers in PubMed, 333 citations in OpenAlex.
- Evolutionary persistence of a highly prevalent multicopy mitochondrial-derived nuclear insertion (Mega-NUMT) in Neotropical Drosophila flies.Molecular biology and evolution · 2026Article
- Ghosts of symbionts past: the hidden history of the dynamic association between filarial nematodes and their Wolbachia endosymbionts.G3 (Bethesda, Md.) · 2025Article
- Recent horizontal transfer of transposable elements inbioRxiv : the preprint server for biology · 2025Article
- Facultative EndosymbiontPlants (Basel, Switzerland) · 2025Article
- Genomes of nitrogen-fixing eukaryotes reveal an alternate path for organellogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Continuously high Wolbachia incidence in flea populations may result from dual-strain infections with divergent effects.Scientific reports · 2025Article
- Review
- Suppression of cytoplasmic incompatibility in the leaf-mining flyRoyal Society open science · 2025Article
- Addictive manipulation: a perspective on the role of reproductive parasitism in the evolution of bacteria-eukaryote symbioses.Biology letters · 2024Review
- Acquired stress resilience through bacteria-to-nematode interdomain horizontal gene transfer.The EMBO journal · 2023Article
- Unraveling the complex evolutionary history of lepidopteran chromosomes through ancestral chromosome reconstruction and novel chromosome nomenclature.BMC biology · 2023Article
- Transinfected Wolbachia strains induce a complex of cytoplasmic incompatibility phenotypes: Roles of CI factor genes.Environmental microbiology reports · 2023Article
- Genomic Assessment of the Contribution of theInternational journal of molecular sciences · 2023Article
- Article
- When does the female bias arise? Insights from the sex determination cascade of a flea beetle with a strongly skewed sex ratio.Functional & integrative genomics · 2023Article
- Characterization of the Bacterial Microbiome in Natural Populations of Barley Stem Gall Midge,Microorganisms · 2023Article
- Distribution of Bacterial Endosymbionts of theInternational journal of molecular sciences · 2023Article
- Genome-wide detection ofFrontiers in cellular and infection microbiology · 2023Article
- Remnants of horizontal transfers of Wolbachia genes in a Wolbachia-free woodwasp.BMC ecology and evolution · 2022Article
- Positive Selection and Horizontal Gene Transfer in the Genome of a Male-Killing Wolbachia.Molecular biology and evolution · 2022Article
68 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adzuki bean beetle, Callosobruchus chinensis, is triple-infected with distinct lineages of Wolbachia endosymbiont, wBruCon, wBruOri, and wBruAus, which were identified by their wsp (Wolbachia surface protein) gene sequences. Whereas wBruCon and wBruOri caused cytoplasmic incompatibility of the host insect, wBruAus did not. Although wBruCon and wBruOri were easily eliminated by antibiotic treatments, wBruAus persisted over five treated generations and could not be eliminated. The inheritance pattern of wBruAus was, surprisingly, explained by sex-linked inheritance in male-heterozygotic organisms, which agreed with the karyotype of C. chinensis (2n = 20, XY). Quantitative PCR analysis demonstrated that females contain around twice as much wsp titer as males, which is concordant with an X chromosome linkage. Specific PCR and Southern blot analyses indicated that the wBruAus-bearing strain of C. chinensis contains only a fraction of the Wolbachia gene repertoire. Several genome fragments of wBruAus were isolated using an inverse PCR technique. The fragments exhibited a bacterial genome structure containing a number of ORFs typical of the alpha-proteobacteria, although some of the ORFs contained disruptive mutations. In the flanking region of ftsZ gene, a non-long terminal repeat (non-LTR) retrotransposon sequence, which is typical of insects but not found from bacteria, was present. These results strongly suggest that wBruAus has no microbial entity but is a genome fragment of Wolbachia endosymbiont transferred to the X chromosome of the host insect.
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