ArticleNucleic acids research2002
A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.Biochemistry research international · 2024Review
- Synthesis and Structure-Activity relationships of cyclin-dependent kinase 11 inhibitors based on a diaminothiazole scaffold.European journal of medicinal chemistry · 2022Article
- Rational Design of an Activatable Reporter for Quantitative Imaging of RNA Aberrant SplicingMolecular therapy. Methods & clinical development · 2020Article
- C3G dynamically associates with nuclear speckles and regulates mRNA splicing.Molecular biology of the cell · 2018Article
- Alteration of RNA Splicing by Small-Molecule Inhibitors of the Interaction between NHP2L1 and U4.SLAS discovery : advancing life sciences R & D · 2018Article
- Article
- Intragenic Enhancers Attenuate Host Gene Expression.Molecular cell · 2017Article
- Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing.The Journal of biological chemistry · 2014Article
- Article
- A high-throughput assay to identify small-molecule modulators of alternative pre-mRNA splicing.Journal of biomolecular screening · 2013Article
- Crosstalk between Wnt Signaling and RNA Processing in Colorectal Cancer.Journal of Cancer · 2013Article
- Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.Cell · 2012Article
- The RNA binding motif protein 15B (RBM15B/OTT3) is a functional competitor of serine-arginine (SR) proteins and antagonizes the positive effect of the CDK11p110-cyclin L2α complex on splicing.The Journal of biological chemistry · 2011Article
- Y65C missense mutation in the WW domain of the Golabi-Ito-Hall syndrome protein PQBP1 affects its binding activity and deregulates pre-mRNA splicing.The Journal of biological chemistry · 2010Article
- Synthetic mRNA splicing modulator compounds with in vivo antitumor activity.Journal of medicinal chemistry · 2009Article
- Identification of an exonic splicing silencer in exon 6A of the human VEGF gene.BMC molecular biology · 2009Article
- Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21).The Journal of biological chemistry · 2009Article
- The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing.The Journal of biological chemistry · 2008Article
- ATPase/helicase activities of p68 RNA helicase are required for pre-mRNA splicing but not for assembly of the spliceosome.Molecular and cellular biology · 2005Article
- A dual-light reporter system to determine the efficiency of protein-protein interactions in mammalian cells.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Current methods for measuring the efficiency of splicing in mammalian cells rely on either direct analysis of the RNA, which does not lend itself to rapid assays, or on single reporter functions that are subject to numerous intrinsic variables. If two protein activities are encoded within a single reading frame but on separate exons, with an intervening sequence containing termination codons, then the expression of the second activity is dependent on removal of the intervening sequence by pre-mRNA splicing. Thus, the ratio of the activities encoded by exon 2 to exon 1 reflects the ratio of expression from spliced mRNA to the total expression of spliced and unspliced RNA. This provides a rapid and convenient assay for the effects on splicing efficiency of trans-acting factors or of alterations in the sequences of the intron and surrounding exon sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.