ArticleNucleic acids research2002
Thermodynamic characterization of the multivalent binding of chartreusin to DNA.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 71 citations in OpenAlex.
- Studying the Interaction between Bendamustine and DNA Molecule with SERS Based on AuNPs/ZnClInternational journal of molecular sciences · 2023Article
- Biolayer interferometry provides a robust method for detecting DNA binding small molecules in microbial extracts.Analytical and bioanalytical chemistry · 2021Article
- A polymeric approach toward resistance-resistant antimicrobial agent with dual-selective mechanisms of action.Science advances · 2021Article
- Shape readout of AT-rich DNA by carbohydrates.Biopolymers · 2014Article
- A structural insight into major groove directed binding of nitrosourea derivative nimustine with DNA: a spectroscopic study.PloS one · 2014Article
- General analysis of competitive binding in drug-interceptor-DNA systems.European biophysics journal : EBJ · 2012Article
- Water-mediated binding of agents that target the DNA minor groove.Journal of the American Chemical Society · 2011Article
- Evaluation of molecular descriptors for antitumor drugs with respect to noncovalent binding to DNA and antiproliferative activity.BMC pharmacology · 2009Article
- Influence of substituent modifications on the binding of 2-amino-1,8-naphthyridines to cytosine opposite an AP site in DNA duplexes: thermodynamic characterization.Nucleic acids research · 2009Article
- Heterocyclic diamidine interactions at AT base pairs in the DNA minor groove: effects of heterocycle differences, DNA AT sequence and length.The journal of physical chemistry. B · 2008Article
- Sequence and length dependent thermodynamic differences in heterocyclic diamidine interactions at AT base pairs in the DNA minor groove.Biophysical chemistry · 2007Article
- Entropically-driven binding of mithramycin in the minor groove of C/G-rich DNA sequences.Nucleic acids research · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterization of the thermodynamics of DNA- drug interactions is a very useful part in rational drug design. Isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC) and UV melting experiments have been used to analyze the multivalent (intercalation plus minor groove) binding of the antitumor antibiotic chartreusin to DNA. Using DNA UV melting studies in the presence of the ligand and the binding enthalpy determined by ITC, we determined that the binding constant for the interaction was 3.6 x 10(5) M(-1) at 20 degrees C, in a solution containing 18 mM Na(+). The DNA-drug interaction was enthalpy driven, with a DeltaH(b) of -7.07 kcal/mol at 20 degrees C. Binding enthalpies were determined by ITC in the 20-35 degrees C range and used to calculate a binding-induced change in heat capacity (DeltaCp) of -391 cal/mol K. We have obtained a detailed thermodynamic profile for the interaction of this multivalent drug, which makes possible a dissection of DeltaG(obs) into the component free energy terms. The hydrophobic transfer of the chartreusin chromophore from the solution to the DNA intercalating site is the main contributor to the free energy of binding.
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