ArticleNucleic acids research2002
Analyses of single-copy Arabidopsis T-DNA-transformed lines show that the presence of vector backbone sequences, short inverted repeats and DNA methylation is not sufficient or necessary for the induction of transgene silencing.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation.International journal of molecular sciences · 2020Article
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
- Stochasticity in transcriptional expression of a negative regulator of Arabidopsis ABA network.3 Biotech · 2019Article
- A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae).Molecules (Basel, Switzerland) · 2017Article
- Agrobacterium tumefaciens-mediated transformation of the lichen fungus, Umbilicaria muehlenbergii.PloS one · 2013Article
- Formation of complex extrachromosomal T-DNA structures in Agrobacterium tumefaciens-infected plants.Plant physiology · 2012Article
- Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.Molecular biotechnology · 2011Article
- Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation.Plant cell reports · 2011Article
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA.Transgenic research · 2009Article
- High frequency Agrobacterium tumefaciens-mediated plant transformation induced by ammonium nitrate.Plant cell reports · 2009Article
- Microhomologies between T-DNA ends and target sites often occur in inverted orientation and may be responsible for the high frequency of T-DNA-associated inversions.Plant cell reports · 2007Article
- Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants.The Plant cell · 2006Article
- Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation.Plant cell reports · 2006Article
- An inverted repeat transgene with a structure that cannot generate double-stranded RNA, suffers silencing independent of DNA methylation.Transgenic research · 2006Article
- Number and accuracy of T-DNA insertions in transgenic banana (Musa spp.) plants characterized by an improved anchored PCR technique.Transgenic research · 2006Article
- The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco.Nucleic acids research · 2006Article
- Active RNA silencing at low temperature indicates distinct pathways for antisense-mediated gene-silencing in potato.Plant molecular biology · 2005Article
- The Bt gene cry2Aa2 driven by a tissue specific ST-LS1 promoter from potato effectively controls Heliothis virescens.Transgenic research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In genetically transformed plants, transgene silencing has been correlated with multiple and complex insertions of foreign DNA, e.g. T-DNA and vector backbone sequences. Occasionally, single-copy transgenes also suffer transgene silencing. We have compared integration patterns and T-DNA/plant DNA junctions in a collection of 37 single-copy T-DNA-transformed Arabidopsis lines, of which 13 displayed silencing. Vector sequences were found integrated in five lines, but only one of these displayed silencing. Truncated T-DNA copies, positioned in inverse orientation to an intact T-DNA copy, were discovered in three lines. The whole nptII gene with pnos promoter was present in the truncated copy of one such line in which heavy silencing has been observed. In the two other lines no silencing has been observed over five generations. Thus, vector sequences and short additional T-DNA sequences are not sufficient or necessary to induce transgene silencing. DNA methylation of selected restriction endonuclease sites could not be correlated with silencing. Our collection of T-DNA/plant DNA junctions has also been used to evaluate current models of T-DNA integration. Data for some of our lines are compatible with T-DNA integration in double-strand breaks, while for others initial invasion of plant DNA by the left or by the right T-DNA end seem important.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.