ArticleNucleic acids research2002
Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 175 papers.
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175 citing papers in PubMed, 312 citations in OpenAlex.
- Emerging roles of tRNA modification-mediated codon-specific translational reprogramming in cancer biology.Cell death & disease · 2026Review
- Comparative analysis of m6A mRNA modification inFrontiers in genetics · 2026Article
- The Emerging Role of METTL3 in Lung Diseases.International journal of molecular sciences · 2025Review
- Multiplex metabolic engineering of yeast for high-efficiency all-trans-retinoic acid production.Microbial cell factories · 2025Article
- mInternational journal of molecular sciences · 2025Article
- mRNA mGenome biology · 2025Article
- The roles of a MiRNA and its targeted methyltransferase 3 in carotenoid accumulation in adductor muscles of QN orange scallops.BMC genomics · 2025Article
- Small Nucleolar RNAs and the Brain: Growing Evidence Supporting Their Role in Psychiatric Disorders.Biological psychiatry global open science · 2025Review
- ALBA proteins facilitate cytoplasmic YTHDF-mediated reading of m6A in Arabidopsis.The EMBO journal · 2024Article
- The Role of N6-methyladenosine Modification in Gametogenesis and Embryogenesis: Impact on Fertility.Genomics, proteomics & bioinformatics · 2024Review
- Structures and mechanisms of the RNA mActa biochimica et biophysica Sinica · 2024Review
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- NAging and disease · 2024Review
- Insights into the role of RNA mGenes & diseases · 2024Review
- Understanding the redundant functions of the mRNA (New York, N.Y.) · 2024Article
- Phylogenetic and functional analyses ofmSphere · 2024Article
- Sinefungin, a natural nucleoside analog of S-adenosyl methionine, impairs the pathogenicity ofnpj antimicrobials and resistance · 2024Article
- The mFrontiers in pharmacology · 2024Review
- Intestinal transit-amplifying cells require METTL3 for growth factor signaling and cell survival.JCI insight · 2023Article
- Rie1 and Sgn1 form an RNA-binding complex that enforces the meiotic entry cell fate decision.The Journal of cell biology · 2023Article
115 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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Abstract
N6-methyladenosine (m6A) is present at internal sites in mRNA isolated from all higher eukaryotes, but has not previously been detected in the mRNA of the yeast Saccharomyces cerevisiae. This nucleoside modification occurs only in a sequence- specific context that appears to be conserved across diverse species. The function of this modification is not fully established, but there is some indirect evidence that m6A may play a role in the efficiency of mRNA splicing, transport or translation. The S.cerevisiae gene IME4, which is important for induction of sporulation, is very similar to the human gene MT-A70, which has been shown to be a critical subunit of the human mRNA [N6-adenosine]-methyltransferase. This observation led to the hypothesis that yeast sporulation may be dependent upon methylation of yeast mRNA, mediated by Ime4p. In this study we show that induction of sporulation leads to the appearance of low levels of m6A in yeast mRNA and that this modification requires IME4. Moreover, single amino acid substitutions in the putative catalytic residues of Ime4p lead to severe sporulation defects in a strain whose sporulation ability is completely dependent on this protein. Collectively, these data suggest very strongly that the activation of sporulation by Ime4p is the result of its proposed methyltransferase activity and provide the most direct evidence to date of a physiologic role of m6A in a gene regulatory pathway.
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