ArticleNucleic acids research2002
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 59 citations in OpenAlex.
- Life stage-specific poly(A) site selection regulated byProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Gel shift experiments with fragments of the Trypanosoma brucei RNA-binding protein RBP10.BMC research notes · 2022Article
- Regulation of early endosomes across eukaryotes: Evolution and functional homology of Vps9 proteins.Traffic (Copenhagen, Denmark) · 2018Article
- Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei.PLoS pathogens · 2017Article
- The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes.Eukaryotic cell · 2014Article
- An AU-rich instability element in the 3'UTR mediates an increase in mRNA stability in response to expression of a dhh1 ATPase mutant.Translation (Austin, Tex.) · 2014Article
- Global identification of conserved post-transcriptional regulatory programs in trypanosomatids.Nucleic acids research · 2013Article
- Cell density-dependent ectopic expression in bloodstream form Trypanosoma brucei.Experimental parasitology · 2013Article
- Rab28 function in trypanosomes: interactions with retromer and ESCRT pathways.Journal of cell science · 2011Article
- The role of the 5'-3' exoribonuclease XRNA in transcriptome-wide mRNA degradation.RNA (New York, N.Y.) · 2011Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- Transcriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons.BMC genomics · 2009Article
- The trypanosome Rab-related proteins RabX1 and RabX2 play no role in intracellular trafficking but may be involved in fly infectivity.PloS one · 2009Article
- The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.Nucleic acids research · 2009Article
- Differential expression of a protease gene family in African trypanosomes.Molecular and biochemical parasitology · 2009Article
- The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes.Nucleic acids research · 2008Article
- A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells.Nucleic acids research · 2008Article
- Deadenylation-independent stage-specific mRNA degradation in Leishmania.Nucleic acids research · 2008Article
- Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes.Eukaryotic cell · 2007Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a more developed mitochondrion. We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA in bloodstream forms. The human HuR protein binds to, and stabilises, mammalian mRNAs containing AREs. Expression of HuR in bloodstream-form trypanosomes resulted in growth arrest and in stabilisation of the EP, PGKB and pyruvate, phosphate dikinase mRNAs, while three bloodstream-specific mRNAs were reduced in abundance. The synthesis and abundance of unregulated mRNAs and proteins were unaffected. Our results suggest that regulation of mRNA stability by AREs arose early in eukaryotic evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.