ArticleProceedings of the National Academy of Sciences of the United States of America2002
Karyopherin beta 2B participates in mRNA export from the nucleus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Nuclear transport proteins: structure, function, and disease relevance.Signal transduction and targeted therapy · 2023Review
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Nucleocytoplasmic Transport: Regulatory Mechanisms and the Implications in Neurodegeneration.International journal of molecular sciences · 2021Review
- Transportin-1: A Nuclear Import Receptor with Moonlighting Functions.Frontiers in molecular biosciences · 2021Review
- Reduction of mRNA export unmasks different tissue sensitivities to low mRNA levels during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Molecular and ultrastuctural changes of rat pre-implantation embryos during two-cell developmental arrest.Journal of assisted reproduction and genetics · 2014Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F.PloS one · 2013Article
- Importins and exportins in cellular differentiation.Journal of cellular and molecular medicine · 2008Review
- Transportins 1 and 2 are redundant nuclear import factors for hnRNP A1 and HuR.RNA (New York, N.Y.) · 2004Article
- Nucleocytoplasmic transport: integrating mRNA production and turnover with export through the nuclear pore.Molecular and cellular biology · 2004Review
- Transportin2 functions as importin and mediates nuclear import of HuR.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export.Molecular and cellular biology · 2004Article
- Nucleo-cytoplasmic partitioning of proteins in plants: implications for the regulation of environmental and developmental signalling.Current genetics · 2003Review
- Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7.Plant molecular biology · 2003Article
- An essential role for hGle1 nucleocytoplasmic shuttling in mRNA export.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transport of macromolecules between the cell nucleus and cytoplasm occurs through the nuclear pores and is mediated by soluble carriers known as karyopherins (Kaps), transportins, importins, or exportins. We report that Kap beta2B (transportin-2) forms complexes with the mRNA export factor TAP in the presence of RanGTP, as shown by coimmunoprecipitation from HeLa cells. The interaction strictly depends on the presence of RanGTP. In digitonin-permeabilized cells, Kap beta2B mediates TAP-GFP export from the nuclei in the presence of RanGTP. A TAP mutant that does not coimmunoprecipitate with Kap beta2B is also not exported by Kap beta2B. In the permeabilized cells assay, TAP is also exported independently of Kap beta2B by direct interaction with nucleoporins, in agreement with previous reports. The export rate is, however, significantly lower than the Kap beta2B-mediated pathway. Both Kap beta2B and TAP are present and enriched in the poly(A)(+) RNA complexes isolated from HeLa cell nuclear lysates. Poly(A)(+) RNA strongly accumulates in the nuclei of HeLa cells treated with Kap beta2B short interfering RNA, indicating that Kap beta2B is involved in the export of at least a large proportion of the mRNA species. The export of beta-actin and GAPDH mRNA is also inhibited, whereas 28S RNA is not affected. The data support the conclusion that Kap beta2B participates directly in the export of a large proportion of cellular mRNAs, and TAP connects Kap beta2B to the mRNAs to be exported.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.