ArticleInternational journal of experimental pathology2002
Mechanisms of 5-azacytidine (5AzC)-induced toxicity in the rat foetal brain.
Article in International journal of experimental pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 27 citations in OpenAlex.
- The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor.Epigenetics & chromatin · 2021Article
- A single day of 5-azacytidine exposure during development induces neurodegeneration in neonatal mice and neurobehavioral deficits in adult mice.Physiology & behavior · 2016Article
- Effect of methotrexate on neuroepithelium in the rat fetal brain.The Journal of veterinary medical science · 2014Article
- Epigenetic medicine and fetal alcohol spectrum disorders.Epigenomics · 2013Review
- Distribution and sequence of pyknotic cells in rat fetuses exposed to busulfan.Journal of toxicologic pathology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Mechanisms of 5-azacytidine (5AzC)-induced toxicity in the rat foetal brain were investigated. 5AzC (10 mg/kg) was injected into pregnant rats on day 13 of gestation and the protein and mRNA expressions of p53 and its transcriptional target genes, p21, bax, cyclin G1, fas, and gadd45, were examined in the foetal brain. The number of p53-positive cells peaked at 9 h after treatment (HAT) and those of apoptotic cells and p21-positive cells peaked at 12 HAT. The expressions of p21, bax, cyclin G1, and fas mRNAs were significantly elevated from 9 to 12 HAT. From the experiments using 5-bromo-2'-deoxyuridine (BrdU), as compared with controls, the migration of neuroepithelial cells significantly delayed and BrdU-positive signals were observed in many apoptotic cells from 9 to 24 HAT in the 5AzC-group. In addition, the number of S phase cells significantly decreased at 12 HAT. The present results indicate that 5AzC induced apoptosis and cell cycle arrest probably at G1 phase in the rat foetal brain and they might be mediated by p53 in response to DNA damage.
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