ArticleProceedings of the National Academy of Sciences of the United States of America2002
Tubulin is the endogenous inhibitor of the glyceraldehyde 3-phosphate dehydrogenase isoform that catalyzes membrane fusion: Implications for the coordinated regulation of glycolysis and membrane fusion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- Insights in tubulin-like structures of the poultry pathogen Mycoplasmoides gallisepticum.Archives of microbiology · 2026Article
- Rational engineering unlocks the therapeutic potential of WHP1: A revolutionary peptide poised to advance wound healing.PloS one · 2025Article
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- Cytonemes Versus Neutrophil Extracellular Traps in the Fight of Neutrophils with Microbes.International journal of molecular sciences · 2020Review
- Identification of Bacterial Protein Interaction Partners Points to New Intracellular Functions ofFrontiers in microbiology · 2020Article
- AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Identification of host cellular targets of AC4 and AV2 proteins of tomato leaf curl palampur virus and their sub-cellular localization studies.Virusdisease · 2017Article
- An Emerging Role for Tubulin Isotypes in Modulating Cancer Biology and Chemotherapy Resistance.International journal of molecular sciences · 2017Review
- Engineering of soybean mosaic virus as a versatile tool for studying protein-protein interactions in soybean.Scientific reports · 2016Article
- Molecular association of glucose-6-phosphate isomerase and pyruvate kinase M2 with glyceraldehyde-3-phosphate dehydrogenase in cancer cells.BMC cancer · 2016Article
- Critical protein GAPDH and its regulatory mechanisms in cancer cells.Cancer biology & medicine · 2015Review
- Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage.Molecular brain · 2014Article
- Microtubules and their role in cellular stress in cancer.Frontiers in oncology · 2014Review
- Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection.Retrovirology · 2012Article
- Externalized glycolytic enzymes are novel, conserved, and early biomarkers of apoptosis.The Journal of biological chemistry · 2012Article
- Glyceraldehyde 3-phosphate dehydrogenase negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA.Journal of virology · 2011Article
- The diverse functions of GAPDH: views from different subcellular compartments.Cellular signalling · 2011Review
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interaction with 3' ends of Japanese encephalitis virus RNA and colocalization with the viral NS5 protein.Journal of biomedical science · 2009Article
- Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previously we demonstrated that specific chromatographically resolvable isoforms of rabbit brain GAPDH catalyze either glycolytic flux or membrane fusion activity (but not both). Moreover, GAPDH membrane fusion activity was latent until it was separated from an endogenous cytosolic inhibitor by anion-exchange chromatography. Herein we demonstrate that the cytosolic inhibitor is nondialyzable, heat-labile, and trypsin-sensitive, thereby identifying it as a cytosolic protein constituent. Chromatographic purification of the rabbit-brain cytosolic protein inhibitor of GAPDH isoform-catalyzed membrane fusion identified a predominant 55-kDa doublet that contained an internal 15-aa peptide identical to a sequence present in alpha-tubulin (residues 65-79). The identity of the 55-kDa doublet as tubulin was substantiated through Western blot analysis and inhibition of GAPDH-catalyzed membrane fusion by authentic tubulin. Stopped-flow kinetic analysis demonstrated the high-affinity, rapid, and direct modulation of GAPDH-catalyzed fusion activity by tubulin. Because GTP-activated Rab 2 recruits GAPDH to membranes about to undergo fusion [Tisdale, E. J. (2001) J. Biol. Chem. 276, 2480-2486] and protein kinase Ciota/lambda phosphorylates GAPDH modulating its interactions with tubulin [Tisdale, E. J. (2001) J. Biol. Chem. 277, 3334-3341], the present study suggests a coordinated mechanism through which membrane trafficking and cellular signaling can be integrated with glycolytic flux.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.