Evidence map›Paper›PMID 12381357›Full record

ArticleAnalytical biochemistry2002

Real-time background suppression during frequency domain lifetime measurements.

Petr Herman, Badri P Maliwal, Joseph R Lakowicz, Baldri P Maliwal

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

  • Erratum issued
    Maliwal, Baldri P [corrected to Maliwal, Badri P]
5 · Who and what money

Authors and funding

4 authors.

Petr HermanCenter for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.
Badri P Maliwal
Joseph R Lakowicz
Baldri P Maliwal

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119
6 · The paper itself

Abstract

We describe real time background suppression of autofluorescence from biological samples during frequency domain or phase modulation measurements of intensity decays. For these measurements the samples were excited with a train of light pulses with widths below 1 ps. The detector was gated off for a short time period of 10 to 40 ns during and shortly after the excitation pulse. The reference signal needed for the frequency domain measurement was provided by a long-lifetime reference fluorophore which continues to emit following the off-gating pulse. Both the sample and the reference were measured under identical optical and electronic conditions avoiding the need for correction of the photomultiplier tube signal for the gating sequence. We demonstrate frequency domain background suppression using a mixture of short- and long-lifetime probes and for a long-lifetime probe in human plasma with significant autofluorescence.

Indexed as

2,2'-DipyridylComputer SimulationFluorescenceFluorometryFourier AnalysisHumansOrganometallic CompoundsSensitivity and SpecificitySpectrometry, FluorescenceTime FactorsXanthenes2,2'-DipyridylOrganometallic Compoundsruthenium bis(bipyridine)dicarboxybipyridineTexas redXanthenes

Identifiers

PMID12381357
PMCPMC6945983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.