ArticleThe Journal of cell biology2002
Cytosolic free Ca(2+) changes and calpain activation are required for beta integrin-accelerated phagocytosis by human neutrophils.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 115 citations in OpenAlex.
- Intraphagosomal Free CaInternational journal of molecular sciences · 2024Article
- The formation and function of the neutrophil phagosome.Immunological reviews · 2023Review
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.Biophysical journal · 2022Article
- Neutrophil signaling during myocardial infarction wound repair.Cellular signalling · 2021Review
- The GPR120 Agonist TUG-891 Inhibits the Motility and Phagocytosis of Mouse Alveolar Macrophages.BioMed research international · 2020Article
- Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis.International journal of molecular sciences · 2019Review
- Mechanistic Understanding of Single-Cell Behavior is Essential for Transformative Advances in Biomedicine.The Yale journal of biology and medicine · 2018Review
- Mechanistic Role of the Calcium-Dependent Protease Calpain in the Endothelial Dysfunction Induced by MPO (Myeloperoxidase).Hypertension (Dallas, Tex. : 1979) · 2018Article
- Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts.Science signaling · 2018Article
- Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading.Scientific reports · 2017Article
- Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux.Cell reports · 2015Article
- Conformationally restricted calpain inhibitors.Chemical science · 2015Article
- Ca²⁺ and calpain control membrane expansion during the rapid cell spreading of neutrophils.Journal of cell science · 2013Article
- New insights into the regulation of neutrophil NADPH oxidase activity in the phagosome: a focus on the role of lipid and Ca(2+) signaling.Antioxidants & redox signaling · 2013Review
- Redox signal-mediated sensitization of transient receptor potential melastatin 2 (TRPM2) to temperature affects macrophage functions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The Drosophila TRPP cation channel, PKD2 and Dmel/Ced-12 act in genetically distinct pathways during apoptotic cell clearance.PloS one · 2012Article
- Blurred line between chemotactic chase and phagocytic consumption: an immunophysical single-cell perspective.Journal of cell science · 2011Review
- Target-specific mechanics of phagocytosis: protrusive neutrophil response to zymosan differs from the uptake of antibody-tagged pathogens.Journal of cell science · 2011Article
- β2 integrin induces TCRζ-Syk-phospholipase C-γ phosphorylation and paxillin-dependent granule polarization in human NK cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis of microbes coated with opsonins such as the complement component C3bi is the key activity of neutrophils. However, the mechanism by which opsonins enhance the rate of phagocytosis by these cells is unknown and has been difficult to study, partly because of the problem of observing and quantifying the events associated with phagocytosis. In this study, C3bi-opsonized particles were presented to neutrophils with a micromanipulator, so that the events of binding, pseudopod cup formation, engulfment, and completion of phagocytosis were clearly defined and distinguished from those involved with chemotaxis. Using this approach in combination with simultaneous phase contrast and Ca(2+) imaging, the temporal relationship between changes in cytosolic free Ca(2+) concentration and phagocytosis were correlated. Here we show that whereas small, localized Ca(2+) changes occur at the site of particle attachment and cup formation as a result of store release, rapid engulfment of the particle required a global change in cytosolic free Ca(2+) which resulted from Ca(2+) influx. This latter rise in cytosolic free Ca(2+) concentration also liberated a fraction of beta2 integrin receptors which were initially immobile on the neutrophil surface, as demonstrable by both fluorescence recovery after laser bleaching and by visualization of localized beta2 integrin labelling. Inhibitors of calpain activation prevented both the Ca(2+)-induced liberation of beta2 integrin and the rapid stage of phagocytosis, despite the persistence of the global Ca(2+) signal. Therefore, we propose that Ca(2+) activation of calpain causes beta2 integrin liberation, and that this signal plays a key role in the acceleration of beta2 integrin-mediated phagocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.