ArticleProceedings of the National Academy of Sciences of the United States of America2002
Hematopoietic-specific activators establish an overlapping pattern of histone acetylation and methylation within a mammalian chromatin domain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- Epigenetic and Transcriptional Control of Erythropoiesis.Frontiers in genetics · 2022Review
- p45 NF-E2 regulates syncytiotrophoblast differentiation by post-translational GCM1 modifications in human intrauterine growth restriction.Cell death & disease · 2017Article
- Altered chromatin occupancy of master regulators underlies evolutionary divergence in the transcriptional landscape of erythroid differentiation.PLoS genetics · 2014Article
- Hematopoietic transcriptional mechanisms: from locus-specific to genome-wide vantage points.Experimental hematology · 2014Review
- Epigenetic and genetic mechanisms in red cell biology.Current opinion in hematology · 2014Review
- The hematopoietic regulator TAL1 is required for chromatin looping between the β-globin LCR and human γ-globin genes to activate transcription.Nucleic acids research · 2014Article
- Transcriptional mechanisms underlying hemoglobin synthesis.Cold Spring Harbor perspectives in medicine · 2013Review
- Article
- Master regulatory GATA transcription factors: mechanistic principles and emerging links to hematologic malignancies.Nucleic acids research · 2012Review
- A novel murine model of myeloproliferative disorders generated by overexpression of the transcription factor NF-E2.The Journal of experimental medicine · 2012Article
- Article
- Molecular determinants for small Maf protein control of platelet production.Molecular and cellular biology · 2011Article
- Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Multiple functions of Ldb1 required for beta-globin activation during erythroid differentiation.Blood · 2010Article
- Influence of immune privilege on ocular tumor development.Ocular immunology and inflammation · 2010Review
- Article
- Differential requirement of a distal regulatory region for pre-initiation complex formation at globin gene promoters.Nucleic acids research · 2009Article
- Epigenetics of beta-globin gene regulation.Mutation research · 2008Review
- The role of transcriptional activator GATA-1 at human beta-globin HS2.Nucleic acids research · 2008Article
- USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.Molecular and cellular biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Posttranslational modification of histones through acetylation, methylation, and phosphorylation is a common mode of regulating chromatin structure and, therefore, diverse nuclear processes. One such modification, methylated histone H3 at lysine-4 (H3-meK4), colocalizes with hyperacetylated histones H3 and H4 in mammalian chromatin. Whereas activators directly recruit acetyltransferases, the process whereby H3-meK4 is established is unknown. We tested whether the hematopoietic-specific activators NF-E2 and GATA-1, which mediate transactivation of the beta-globin genes, induce both histone acetylation and H3-meK4. Through the use of NF-E2- and GATA-1-null cell lines, we show that both activators induce H3 acetylation at the promoter upon transcriptional activation. However, analysis of H3-mek4 revealed that NF-E2 and GATA-1 differentially regulate chromatin modifications at the betamajor promoter. NF-E2, but not GATA-1, induces H3-meK4 at the promoter. Thus, under conditions in which NF-E2 and GATA-1 activate the transcription of an endogenous gene at least 570-fold, these activators differ in their capacity to induce H3-meK4. Despite strong H3-meK4 at hypersensitive site 2 of the upstream locus control region, neither factor was required to establish H3-meK4 at this site. These results support a model in which multiple tissue-specific activators collectively function to assemble a composite histone modification pattern, consisting of overlapping histone acetylation and methylation. As GATA-1 induced H3 acetylation, but not H3-meK4, at the promoter, H3 acetylation and H3-meK4 components of a composite histone modification pattern can be established independently.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.