ArticleInfection and immunity2002
Modulation of inducible nitric oxide synthase expression by the attaching and effacing bacterial pathogen citrobacter rodentium in infected mice.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 97 citations in OpenAlex.
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- Involvement of nitrergic neurons in colonic motility in a rat model of ulcerative colitis.World journal of gastroenterology · 2022Article
- IRE1α-Driven Inflammation Promotes Clearance of Citrobacter rodentium Infection.Infection and immunity · 2022Article
- Dietary phytate primes epithelial antibacterial immunity in the intestine.Frontiers in immunology · 2022Article
- Commensal segmented filamentous bacteria-derived retinoic acid primes host defense to intestinal infection.Cell host & microbe · 2021Article
- Role of p40FEBS open bio · 2021Article
- Interplay between enterohaemorrhagicEmerging microbes & infections · 2020Article
- Dietary Salt Administration Decreases EnterotoxigenicInternational journal of molecular sciences · 2020Article
- Article
- Citrobacter rodentium-host-microbiota interactions: immunity, bioenergetics and metabolism.Nature reviews. Microbiology · 2019Review
- T-cell derived acetylcholine aids host defenses during enteric bacterial infection with Citrobacter rodentium.PLoS pathogens · 2019Article
- Egg Protein Transferrin-Derived Peptides IRW and IQW RegulateFrontiers in microbiology · 2019Article
- Helminth-induced alterations of the gut microbiota exacerbate bacterial colitis.Mucosal immunology · 2018Article
- Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo.Cell metabolism · 2017Article
- Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium.World journal of microbiology & biotechnology · 2017Article
- The commensal microbiota exacerbate infectious colitis in stressor-exposed mice.Brain, behavior, and immunity · 2017Article
- Social stress-enhanced severity of Citrobacter rodentium-induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri.Mucosal immunology · 2016Article
- Early IFN type I response: Learning from microbial evasion strategies.Seminars in immunology · 2015Review
- Role of GM-CSF in the inflammatory cytokine network that regulates neutrophil influx into the colonic mucosa during Clostridium difficile infection in mice.Gut microbes · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citrobacter rodentium belongs to the attaching and effacing family of enteric bacterial pathogens that includes both enteropathogenic and enterohemorrhagic Escherichia coli. These bacteria infect their hosts by colonizing the intestinal mucosal surface and intimately attaching to underlying epithelial cells. The abilities of these pathogens to exploit the cytoskeleton and signaling pathways of host cells are well documented, but their interactions with the host's antimicrobial defenses, such as inducible nitric oxide synthase (iNOS), are poorly understood. To address this issue, we infected mice with C. rodentium and found that iNOS mRNA expression in the colon significantly increased during infection. Immunostaining identified epithelial cells as the major source for immunoreactive iNOS. Finding that nitric oxide (NO) donors were bacteriostatic for C. rodentium in vitro, we examined whether iNOS expression contributed to host defense by infecting iNOS-deficient mice. Loss of iNOS expression caused a small but significant delay in bacterial clearance without affecting tissue pathology. Finally, immunofluorescence staining was used to determine if iNOS expression was localized to infected cells by staining for the C. rodentium virulence factor, translocated intimin receptor (Tir), as well as iNOS. Interestingly, while more than 85% of uninfected epithelial cells expressed iNOS, fewer than 15% of infected (Tir-positive) cells expressed detectable iNOS. These results demonstrate that both iNOS and intestinal epithelial cells play an active role in host defense during C. rodentium infection. However, the selective expression of iNOS by uninfected but not infected cells suggests that this pathogen has developed mechanisms to locally limit its exposure to host-derived NO.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.